TPDB

TPDB ID TPDB93339

Peptide_Name

Connective tissue-ac

Activity

Blood-Brain Barrier

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

Peptipedia

Sequence_Type

modified_plain

Has_Modification Yes

Description A 16-aa modified blood-brain barrier peptide sequence curated from Peptipedia, without an available 3D structural model.

Modification

C-terminal: Amidation Chemical: Cys2-Pen7

Sequence

fCYwRT-Pen-T-NH2

FASTA

>TPDB93339|Blood-Brain Barrier|length=16
fCYwRT-Pen-T-NH2

Formula C70H94N20O20S1

Absent Amino Acids ADQGILKMSV

Common Amino Acids NT

Mass 1567.70

PI 7.15

Basic Residues 2

Acidic Residues 1

Hydrophobic Residues 3

Net Charge +0

Boman Index 1.20

Hydrophobicity -1.131

Aliphatic Index 0.00

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 6990

Absorbance 280nm 445.88

Polar Residues 6

number

0

A

1

R

2

N

0

D

1

C

1

E

0

Q

0

G

1

H

0

I

0

L

0

K

0

M

1

F

1

P

0

S

2

T

1

W

1

Y

0

V

Amino Acid Distribution

A: 0 R: 1 N: 2 D: 0 C: 1 E: 1 Q: 0 G: 0 H: 1 I: 0 L: 0 K: 0 M: 0 F: 1 P: 1 S: 0 T: 2 W: 1 Y: 1 V: 0

Length

16

Formula

C70H94N20O20S1

Mass

1567.70

PI

7.15

Net Charge

+0

Boman Index

1.20

Hydrophobicity

-1.131

Chemical_Modification

Cys2-Pen7

Conformation

Linear

Peptide_Length

Similarity

Peptide_Sequence_3Letter

(D)Phe-Cys-Tyr-(D)Trp-Arg-Thr-Pen-Thr-NH2

SMILES

O=C(N)C(NC(=O)C2NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(N)Cc1ccccc1)CSSC2(C)C)Cc3ccc(O)cc3)Cc5c4ccccc4nc5)CCCNC(=[N@H])N)C(O)C)C(O)C

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 71.50%

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response % parenchyma

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 28.50%

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response % capillary

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 3 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 60 min

Assay & Model

In Vitro Method HPLC

Animal Model Origin/Source

Response half life, serum

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 4 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 119 min

Assay & Model

In Vitro Method HPLC

Animal Model Origin/Source

Response half life, brain

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 5 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result -0.000023 mL/(g x min)

Assay & Model

Animal Model Origin/Source

Response Unidirectional influx rate constant

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

In Vivo Mode of Delivery Intravenous

Transport Type non saturable

Label Direct iodination

Evidence 6 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.01959 ml/g

Assay & Model

Animal Model Origin/Source

Response Initial disrobution volume

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

In Vivo Mode of Delivery Intravenous

Transport Type non saturable

Label Direct iodination

In_Vivo_Mode_of_Delivery

Intravenous