TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB93254

Peptide_Name

Hph-1-PTD

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0 MLCPP 2.0 Peptipedia PerseuCPP

Sequence_Type

standard

Has_Modification No

Description A 11-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, MLCPP 2.0, Peptipedia, and other sources, with an available 3D structural model.

Modification

N-terminal: Conjugated with FITC via Ahx linker

Sequence

YARVRRRGPRR

FASTA

>TPDB93254|Cell-penetrating Peptides|length=11
YARVRRRGPRR

Formula C60H107N29O13

Absent Amino Acids NDCEQHILKMFSTW

Common Amino Acids R

Mass 1442.70

PI 12.70

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 3

Net Charge +6

Boman Index 4.28

Hydrophobicity -2.209

Aliphatic Index 35.45

Half Life

Mammalian: 2.8 hour Yeast: 10 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 103.28

Polar Residues 1

number

1

A

6

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

0

I

0

L

0

K

0

M

0

F

1

P

0

S

0

T

0

W

1

Y

1

V

Amino Acid Distribution

A: 1 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 1 S: 0 T: 0 W: 0 Y: 1 V: 1

Length

11

Formula

C60H107N29O13

Mass

1442.70

PI

12.70

Net Charge

+6

Boman Index

4.28

Hydrophobicity

-2.209

C_terminal_Modification

Free

Chirality

L

Linearity

Linear

N_terminal_Modification

Conjugated with FITC via Ahx linker

SMILES

N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Bone Marrow Macrophages (Bmms) Isolated From Wt Mice

Assay & Model

In Vitro Model Cre transgene mouse line (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J, JAX Mice

In Vivo Model Cre transgene mouse line (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J, JAX Mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Non-natural residues Linear L

Source of Peptide Protein derived

Cargo Type RANK fragments containing Motif 2 (M2-10)

Subcellular Localization Cytoplasm

In Vitro or In Vivo In vitro and in vivo

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Bone Marrow Macrophages (Bmms) Isolated From Wt Mice

Assay & Model

In Vitro Model Cre transgene mouse line (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J, JAX Mice

In Vivo Model Cre transgene mouse line (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J, JAX Mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type RANK fragments containing Motif 3(M3-10)

Subcellular Localization Cytoplasm

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCSSCCSCC

DSSP_Secondary_Structure

CCCCSSCCSCC