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Peptide record

TPDB93238

RALA Cell-penetrating Peptides standard
30 amino acids
Basic Information
3D PDB MODEL
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TPDB93238
RALA
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 MLCPP 2.0 Peptipedia PerseuCPP
standard
No
A 30-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, MLCPP 2.0, Peptipedia, and other sources, with an available 3D structural model.
Chemical: Nanoparticles
Sequence
WEARLARALARALARHLARALARALRACEA
Physicochemical Analysis
C144H248N54O35S1
NDQGIKMFPSTYV
A
3327.95
12.28
8
2
19
+5
3.18
0.143
118.00
Mammalian: 2.8 hour Yeast: 3 min E.coli: 2 min
5500
165.27
1
Residue Composition
number
12
A
7
R
0
N
0
D
1
C
2
E
0
Q
0
G
1
H
0
I
6
L
0
K
0
M
0
F
0
P
0
S
0
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 12 R: 7 N: 0 D: 0 C: 1 E: 2 Q: 0 G: 0 H: 1 I: 0 L: 6 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0
Chemical Descriptors
30
C144H248N54O35S1
3327.95
12.28
+5
3.18
0.143
Free
Nanoparticles
L
Cationic and amphipathic
Linear
Free
N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)O
Evidence Records 3 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Zr-75–1 Cells

Assay & Model

Female, 6-week-old SCID mice
Female, 6-week-old SCID mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic and amphipathic
Synthetic
pFKBPL and siFKBPL
Cytoplasm and nucleus
FKBPL knockdown and overexpression was observed from 0.125 μg siFKBPL and 1 μg pFKBPL, respectively, when delivered with RALA.
In vitro and in vivo
CCCCCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Hmec-1 Cells

Assay & Model

Skin wounding model in C57BL/6J mice
Skin wounding model in C57BL/6J mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic and amphipathic
Synthetic
siFKBPL
RALA/pEGFP-N1 nanoparticles at a N:P ratio of 6, transfection efficiency was 33.7+_ 1.65% in HMEC-1 endothelial cells
In vitro and in vivo
CCSTTHHHHHHHHHCCHHHHHHTSSCSCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

Hdfs Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic and amphipathic
Synthetic
Dy547-labelled MMP-9-targeting siRNA (siMMP-9)
In normal low glucose the cellular uptake significantly increased from 20.0 ± 3.4% to 66.5 ± 3.1% (p ˂ 0.001), with increasing siRNA concentration from 20 to 80 nmol/L
In vitro
CCSTTHHHHHHHHHCCHHHHHHTSSCSCC
Additional Detail Fields 1 fields
CCCCCCCC CCSTTHHHHHHHHHCCHHHHHHTSSCSCC