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Peptide record

TPDB93237

RALA Cell-penetrating Peptides standard
30 amino acids
Basic Information
3D PDB MODEL
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TPDB93237
RALA
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 MLCPP 2.0 Peptipedia PerseuCPP
standard
No
A 30-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, MLCPP 2.0, Peptipedia, and other sources, with an available 3D structural model.
Chemical: Nanoparticle
Sequence
WEARLARALARALARHLARALARALRACEA
Physicochemical Analysis
C144H248N54O35S1
NDQGIKMFPSTYV
A
3327.95
12.28
8
2
19
+5
3.18
0.143
118.00
Mammalian: 2.8 hour Yeast: 3 min E.coli: 2 min
5500
165.27
1
Residue Composition
number
12
A
7
R
0
N
0
D
1
C
2
E
0
Q
0
G
1
H
0
I
6
L
0
K
0
M
0
F
0
P
0
S
0
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 12 R: 7 N: 0 D: 0 C: 1 E: 2 Q: 0 G: 0 H: 1 I: 0 L: 6 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0
Chemical Descriptors
30
C144H248N54O35S1
3327.95
12.28
+5
3.18
0.143
Free
Nanoparticle
L
Amphipathic Cationic and amphipathic
Linear
Free
N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Dc2.4 Cells

Assay & Model

Female wild type C57BL/6 mice, Ifnar−/− mice, OT-I mice, C57BL/6 luciferase reporter mice (IFN-β+/Δβ-luc)
Female wild type C57BL/6 mice, Ifnar−/− mice, OT-I mice, C57BL/6 luciferase reporter mice (IFN-β+/Δβ-luc)

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Amphipathic
Cy3b-mRNA( eGFP (unmod: L-6301 mod: L-6303) and ovalbumin)
Cytosol
RALA enabling the strongest mRNA uptake, RGSG the lowest, and RRRR an intermediate
Endocytosis
In vitro and in vivo
CCSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

MCF-7 And MDA-MB-231 Breast Cancer Cells Pc-3 And Du145 Prostate Cancer Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Cationic and amphipathic
pEGFP-N1
Transfection efficiencies of RALA (33% in MCF-7 and 31% in MDA-MB-231)
In vitro
Additional Detail Fields 1 fields
CCSCC