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Peptide record

TPDB93222

p5RHH Cell-penetrating Peptides standard
21 amino acids
Basic Information
3D PDB MODEL
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TPDB93222
p5RHH
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 MLCPP 2.0 Peptipedia
standard
No
A 21-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, MLCPP 2.0, and Peptipedia, with an available 3D structural model.
Chemical: Nanoparticles
Sequence
VLTTGLPALISWIRRRHRRHC
Physicochemical Analysis
C112H189N41O25S1
NDEQKMFY
R
2542.05
12.58
7
0
8
+5
2.35
-0.243
111.43
Mammalian: 100 hour Yeast: >20 hour E.coli: >10 hour
5500
216.36
4
Residue Composition
number
1
A
5
R
0
N
0
D
1
C
0
E
0
Q
1
G
2
H
2
I
3
L
0
K
0
M
0
F
1
P
1
S
2
T
1
W
0
Y
1
V
Amino Acid Distribution
A: 1 R: 5 N: 0 D: 0 C: 1 E: 0 Q: 0 G: 1 H: 2 I: 2 L: 3 K: 0 M: 0 F: 0 P: 1 S: 1 T: 2 W: 1 Y: 0 V: 1
Chemical Descriptors
21
C112H189N41O25S1
2542.05
12.58
+5
2.35
-0.243
Free
Nanoparticles
L
Cationic and amphipathic Amphipathic
Linear
Free
N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)O N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CS)C(=O)O N[C@@H](CS)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CS)C(=O)O
Evidence Records 5 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Assay & Model

8-wk-old male C57BL/6J mice
8-wk-old male C57BL/6J mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic and amphipathic
Synthetic
Cy3-labeled p65 siRNAs
NP could be seen penetrating human OA cartilage up to a depth of at least 700 μm, freely diffusing into CHOndrocyte lacunae located in the intermediate zone
In vivo and ex vivo
CCCSSCCCCTTSCSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HUVECs Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic and amphipathic
Synthetic
p65 siRNA
p5RHH-siRNA NP was taken up by the CHOndrocytes but not free Cy3-labeled siRNA. And in HUVECs, Delivery of human p65 siRNA NP led to marked decrease in p65 expression exhibited similar or superior transfection efciency compared with Lipofectamine
In vitro and ex vivo
CCGGGCHHHHHHHHHSSCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

Ark1 And Ovcar8 Cells

Assay & Model

ARK1 and OVCAR8 tumor bearing NOD/SCID and NU/FOX mice model
ARK1 and OVCAR8 tumor bearing NOD/SCID and NU/FOX mice model

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic and amphipathic
Synthetic
Anti-AXL siRNA (siAXL)
Tumors from the IP-injected mice had more intracellular fuorescence than those from IV-injected mice.
In vitro and in vivo
CCGGGCHHHHHHHHHSSCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

B16F10 And Gal8-Yfp Hek293T Cells Casmcs And Haoecs Cells

Assay & Model

Femoral artery wire injury of C57BL6/J mouse model
Femoral artery wire injury of C57BL6/J mouse model

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic and amphipathic
Synthetic
Cy5-uridine-labeled GFP-coding mRNA and niRFP mRNA
Cytoplasm
The expression of niRFP was clearly observed only after 48 h in the wire-injured artery but not in the contralateral uninjured artery of the same niRFP mRNA-p5RHH nanoparticle-treated mice
In vitro and in vivo
CCGGGCHHHHHHHHHSSCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

B16-F10 Cells

Assay & Model

Melanoma lung metastasis mouse model (Male C57BL/C mice)
Melanoma lung metastasis mouse model (Male C57BL/C mice)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphipathic
Protein derived
Anti-NF-kB p65 siRNA
Cytoplasm
24 hours after p5RHH-p65 siRNA nanoparticle treatment p65 mRNA was reduced by 82% and was significantly higher than that of control cells with no treatment (p=6.38e−8), cells treated with p5RHH-scrambled siRNA nanoparticles (p=1.60e−7), and cells treated with free p65 siRNA (p=1.72e−8)
In vitro and in vivo
CCCCCC
Additional Detail Fields 1 fields
CCCSSCCCCTTSCSCC CCGGGCHHHHHHHHHSSCC CCCCCC