TPDB

3D PDB MODEL

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TPDB ID TPDB93167

Peptide_Name

R8 (Alexa) R8

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CellPPD-MOD CPPsite3.0 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 10-aa standard natural cell-penetrating peptides peptide sequence curated from CellPPD-MOD, CPPsite3.0, and Peptipedia, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

RRRRRRRRGC-NH2

FASTA

>TPDB93167|Cell-penetrating Peptides|length=10
RRRRRRRRGC-NH2

Formula C53H106N34O11S1

Absent Amino Acids ANDEQHILKMFPSTWYV

Common Amino Acids R

Mass 1427.71

PI 12.88

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +8

Boman Index 6.25

Hydrophobicity -3.390

Aliphatic Index 0.00

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

0

A

8

R

0

N

0

D

1

C

0

E

0

Q

1

G

0

H

0

I

0

L

0

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 0 C: 1 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

10

Formula

C53H106N34O11S1

Mass

1427.71

PI

12.88

Net Charge

+8

Boman Index

6.25

Hydrophobicity

-3.390

C_terminal_Modification

Amidation

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CS)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Tumor-xenografted nude mice

In Vivo Model Tumor-xenografted nude mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (Alexa660)

Uptake Efficiency Accumulation of R8 was significantly higher than the other peptides in tumors

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCSCTTSCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, T98G glioma cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (Alexa)

Subcellular Localization Cytosol

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSCTTSCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Alexa Fluor 488 dye

Subcellular Localization Cytoplasm

Uptake Mechanism Macropinocytosis and direct penetration

In Vitro or In Vivo In vitro

DSSP_Secondary_Structure

CCCSCTTSCC