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Peptide record

TPDB93165

Cys-R8 R8 Cell-penetrating Peptides standard
9 amino acids
Basic Information
3D PDB MODEL
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TPDB93165
Cys-R8 R8
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 Peptipedia
standard
No
A 9-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0 and Peptipedia, with an available 3D structural model.
C-terminal: Addition of Cysteine
Sequence
RRRRRRRRC
Physicochemical Analysis
C51H103N33O10S1
ANDEQGHILKMFPSTWYV
R
1370.65
12.88
8
0
0
+8
6.24
-3.722
0.00
Mammalian: 1 hour Yeast: 2 min E.coli: 2 min
0
0.00
1
Residue Composition
number
0
A
8
R
0
N
0
D
1
C
0
E
0
Q
0
G
0
H
0
I
0
L
0
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 8 N: 0 D: 0 C: 1 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
9
C51H103N33O10S1
1370.65
12.88
+8
6.24
-3.722
Addition of Cysteine
L
Cationic
Linear
Free
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

A431 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Non-natural residues Linear L Cationic
Synthetic
Liposome and CCK8 peptide
Cytosol
Liposomes modified with both HP (CCK8) and CPP (R8) peptides showed a slightly higher fluorescence intensity than the R8-LPs and CCK8-LPs and higher than the untargeted LPs.
Endocytosis
In vitro
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Caco-2/E12 Co-Cultured Cell Model (Caco-2) And Ht29-Mtx-E12 Cells (E12)

Assay & Model

Diabetic male SD rats (200-220 g, seven weeks old)
Diabetic male SD rats (200-220 g, seven weeks old)

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Cationic
Synthetic
Insulin loaded Phosphoserine (Pho) conjugated PLGA NPs
Uptake of P-R8-Pho NPs on Caco-2 cells was about 3-fold higher than the control P NPs and almost the same level as P-R8 NPs. In E12 cells, P-R8-Pho NPs exhibited 1.65-fold and 3.4-fold higher uptake than that of P-R8 and P NPs, and P-R8 NPs was only 2-fold higher than that of the control P NPs.
In vitro and in vivo