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Peptide record

TPDB93013

cLK peptide Cell-penetrating Peptides standard
32 amino acids
Basic Information
3D PDB MODEL
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TPDB93013
cLK peptide
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 32-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
C-terminal: Amidation N-terminal: Acetylation
Sequence
Ac-LKKLLKLLKKLLKLGGLKKLLKLLKKLLKLAG-NH2
Physicochemical Analysis
C177H336N44O33
RNDCEQHIMFPSTWYV
L
3608.89
11.55
12
0
17
+12
2.78
0.456
198.12
Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min
0
0.00
0
Residue Composition
number
1
A
0
R
0
N
0
D
0
C
0
E
0
Q
3
G
0
H
0
I
16
L
12
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 3 H: 0 I: 0 L: 16 K: 12 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
32
C177H336N44O33
3608.89
11.55
+12
2.78
0.456
Amidation
L
Cyclic
Acetylation
N[C@@H](CC(=O)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)N1CCC[C@H]1C(=O)O
Evidence Records 1 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Human Mesenchymal Stem Cells (Mscs)

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Cyclic L
Synthetic
FAM-labeled miRNA 2861 (FAM-miRNA 2861) and TAMRA-conjugated
Cytoplasm
miRNA 2861 alone showed 2.93% cellular uptake at 50 nM. By contrast, combination of 50 nM FAM-miRNA 2861 and 300 nM ac-LK resulted in 86.5% fluorescence-positive cells and A combination of 50 nM FAM-miRNA 2861 and 600 nM ac-LK resulted in 94.9% fluorescence-positive cells. However, in the lipofectamine group, only 42.6% of cells were positive for fluorescence.
In vitro
CCSCCC
Additional Detail Fields 1 fields
CCSCCC