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Peptide record

TPDB93009

EB-1 EB1 Cell-penetrating Peptides standard
23 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB93009
EB-1 EB1
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CellPPD CellPPD-MOD CPPsite3.0 Peptipedia PerseuCPP
standard
No
A 23-aa standard natural cell-penetrating peptides peptide sequence curated from CellPPD, CellPPD-MOD, CPPsite3.0, and other sources, with an available 3D structural model.
C-terminal: Amidation
Sequence
LIRLWSHLIHIWFQNRRLKWKKK-NH2
Physicochemical Analysis
C150H235N45O27
ADCEGMPTYV
LK
3100.80
12.72
9
0
11
+7
-0.41
-0.630
118.70
Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min
16500
532.12
3
Residue Composition
number
0
A
3
R
1
N
0
D
0
C
0
E
1
Q
0
G
2
H
3
I
4
L
4
K
0
M
1
F
0
P
1
S
0
T
3
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 3 N: 1 D: 0 C: 0 E: 0 Q: 1 G: 0 H: 2 I: 3 L: 4 K: 4 M: 0 F: 1 P: 0 S: 1 T: 0 W: 3 Y: 0 V: 0
Chemical Descriptors
23
C150H235N45O27
3100.80
12.72
+7
-0.41
-0.630
Amidation
L
Amphipathic
Linear
Free
N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)O
Evidence Records 3 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HepG2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Amphipathic
Protein derived
Nucleic acid (siRNA)
Cytosol
Endocytic pathway
In vitro
CCTTTSCCSSSCCCSCSSSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Mges(Enzyme Solution Collected From The Midgut Of S.Gregaria) And S. Gregaria

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Amphipathic
Synthetic
Cys fluorescently labelled dsRNA (dsTub and dsLuc) and siRNA( dsTub siRNA and dsLuc-siRNA)
On feeding EB1:dsRNA, measurement of Tub transcript levels reveald that dsRNA was successfully taken up from the midgut lumen.
In vitro and ex vivo
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

K-562 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphipathic
JOE-labeled bcr-abl siRNA
Cytoplasm and Endosomes
Efficiently mediated siRNA delivery
Dynamin-dependent endocytosis, macropinocytosis and caveola-mediated endocytosis.
In vitro
CTTTSCCCCHHHHHTGGGGTC
Additional Detail Fields 1 fields
CCTTTSCCSSSCCCSCSSSCC CTTTSCCCCHHHHHTGGGGTC