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Peptide record

TPDB92996

shuttle peptide S10 Cell-penetrating Peptides standard
34 amino acids
Basic Information
3D PDB MODEL
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TPDB92996
shuttle peptide S10
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 34-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
Chemical: Glycine-serine linker
Sequence
KWKLARAFARAIKKLGGSGGGSYARALRRQARTG
Physicochemical Analysis
C161H272N58O40
NDCEHMPV
A
3660.30
12.71
10
0
14
+10
2.76
-0.638
66.47
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
6990
190.97
5
Residue Composition
number
7
A
6
R
0
N
0
D
0
C
0
E
1
Q
6
G
0
H
1
I
3
L
4
K
0
M
1
F
0
P
2
S
1
T
1
W
1
Y
0
V
Amino Acid Distribution
A: 7 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 6 H: 0 I: 1 L: 3 K: 4 M: 0 F: 1 P: 0 S: 2 T: 1 W: 1 Y: 1 V: 0
Chemical Descriptors
34
C161H272N58O40
3660.30
12.71
+10
2.76
-0.638
Free
Glycine-serine linker
L
Amphiphilic
Linear
Free
N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)NCC(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)O
Evidence Records 3 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Primary Trachea (Tr) And Intralobar (Ilb) Airway Epithelial Cells

Assay & Model

2-week-old ferret kits and 6–18-month-old adult ferrets
2-week-old ferret kits and 6–18-month-old adult ferrets

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Amphiphilic
Synthetic
GFP protein
S10 peptide was able to mediate GFP protein delivery to ferret basal cells yielding 40.5% cell delivery in the absence of obvious toxicity
In vitro and in vivo
CCCCCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Primary Trachea (Tr) And Intralobar (Ilb) Airway Epithelial Cells

Assay & Model

2-week-old ferret kits and 6–18-month-old adult ferrets
2-week-old ferret kits and 6–18-month-old adult ferrets

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphiphilic
Synthetic
RNPs of Cas9 or Cpf1 (AsCas12a)
S10 shuttle delivery of SpCas9 RNP or Cpf1 RNP to ferret airway basal cells resulted in genome editing efficiencies of 7.57% and 1.41%, respectively,
In vitro and in vivo
CCCTHHHHHHHHTTTSSCSSSHHHHHGGGSCCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

Primary Trachea (Tr) And Intralobar (Ilb) Airway Epithelial Cells

Assay & Model

2-week-old ferret kits and 6–18-month-old adult ferrets
2-week-old ferret kits and 6–18-month-old adult ferrets

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphiphilic
Synthetic
peptide cargo DRI-NLS-Cy5
Delivery efficiency of DRI-NLS-Cy5 increases several fold higher than with GFP protein by S10
In vitro and in vivo
CCCTHHHHHHHHTTTSSCSSSHHHHHGGGSCCCC
Additional Detail Fields 1 fields
CCCCCCCC CCCTHHHHHHHHTTTSSCSSSHHHHHGGGSCCCC