Back to search

Peptide record

TPDB92964

dNP2-cys Blood-Brain Barrier Cell-penetrating Peptides standard
22 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB92964
dNP2-cys
Blood-Brain Barrier Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
BBPpredict CPPsite3.0
standard
No
A 22-aa standard natural multi-activity (Blood-Brain Barrier and Cell-penetrating Peptides) peptide sequence curated from BBPpredict and CPPsite3.0, with an available 3D structural model.
C-terminal: Addition of Cysteine Chemical: CFPE-labelled liposomes (cFd-Lip)
Sequence
KIKKVKKKGRKKIKKVKKKGRK
Physicochemical Analysis
C122H240N42O23
ANDCEQHLMFPSTWY
K
2663.52
12.52
16
0
4
+16
15.02
-2.136
61.82
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
0
0.00
0
Residue Composition
number
0
A
2
R
0
N
0
D
0
C
0
E
0
Q
2
G
0
H
2
I
0
L
14
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
2
V
Amino Acid Distribution
A: 0 R: 2 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 2 L: 0 K: 14 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 2
Chemical Descriptors
22
C122H240N42O23
2663.52
12.52
+16
15.02
-2.136
Addition of Cysteine
CFPE-labelled liposomes (cFd-Lip)
L
Cationic
Linear
Free
N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

bEnd.3 Cells , C6 Cell Line

Assay & Model

C6 glioma bearing BALB/c mice (female, 6-8 weeks old, 20 ± 2 g)
C6 glioma bearing BALB/c mice (female, 6-8 weeks old, 20 ± 2 g)

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Paclitaxel (PTX)
The acid-cleavable Fd-Lip significantly upregulated the uptake (p<0.05 vs cFd-Lip at pH 7.4, p<0.01 vs Fd-Lip). The FA-Lip showed significantly higher uptake than PEG-Lip. Moreover, dual modification of FA and dNP2 further enhanced the cellular uptake, showing synergistic effects.
In vitro and in vivo
CCCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Fr-Positive 4T1 Cells And Fr-Negative Nih-3T3 Cells And In Vitro 3D Spheroids Of 4T1 Cells Or 4T1 And Nih 3T3 Cells

Assay & Model

BALB/c mice (female, 6-8 weeks old, 20 ± 2 g)
BALB/c mice (female, 6-8 weeks old, 20 ± 2 g)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Paclitaxel (PTX)
The endocytosis of cFd-Lip (pH 6.8) was higher than that of dNP2-Lip on folate receptor-positive 4T1 cells. The uptake of cFd-Lip by bEnd.3 monolayer cells was 1.01-, 1.26-, 19.90-, and 23.48-fold higher than that of Fd-Lip, dNP2-Lip, FA-Lip and PEG-Lip, respectively.
Endocytosis
In vitro and in vivo
CCGGGGGGGGGGHHHHTTSCCC
Additional Detail Fields 1 fields
CCCCCC CCGGGGGGGGGGHHHHTTSCCC