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Peptide record

TPDB92947

hLF +4R hLF K7 hLF lin+4R hLF M9A hLF P15A hLF Q6A hLF R13A hLF R13A/P15R hLF R7 hLF R7A hLF R7A/V12R hLF V12R hLF W5A hLF WT Cell-penetrating Peptides standard
22 amino acids
Basic Information
3D PDB MODEL
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TPDB92947
hLF +4R hLF K7 hLF lin+4R hLF M9A hLF P15A hLF Q6A hLF R13A hLF R13A/P15R hLF R7 hLF R7A hLF R7A/V12R hLF V12R hLF W5A hLF WT
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CellPPD CellPPD-MOD CPPsite3.0 MLCPP 2.0 Peptipedia
standard
No
A 22-aa standard natural cell-penetrating peptides peptide sequence curated from CellPPD, CellPPD-MOD, CPPsite3.0, and other sources, with an available 3D structural model.
C-terminal: Amidation N-terminal: Conjugation with 5(6)-Carboxyfluorescein
Sequence
KCFQWQRNMRKVRGPPVSCIKR-NH2
Physicochemical Analysis
C118H197N41O27S3
ADEHLTY
R
2718.30
11.98
7
0
6
+7
4.97
-1.041
44.09
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
5625
206.93
6
Residue Composition
number
0
A
4
R
1
N
0
D
2
C
0
E
2
Q
1
G
0
H
1
I
0
L
3
K
1
M
1
F
2
P
1
S
0
T
1
W
0
Y
2
V
Amino Acid Distribution
A: 0 R: 4 N: 1 D: 0 C: 2 E: 0 Q: 2 G: 1 H: 0 I: 1 L: 0 K: 3 M: 1 F: 1 P: 2 S: 1 T: 0 W: 1 Y: 0 V: 2
Chemical Descriptors
22
C118H197N41O27S3
2718.30
11.98
+7
4.97
-1.041
Amidation
L
Cationic
Linear
Conjugation with 5(6)-Carboxyfluorescein
N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)O
Evidence Records 14 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
High
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Lower than hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Lower than hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Lower than hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Equal to hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Equal to hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Lower than hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 8 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Equal to hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 9 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Equal to hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 10 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Lower than hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 11 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Higher than hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 12 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Higher than hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 13 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Equal to hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Evidence 14 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Jurkat E6.1 leukemia cells, Ovcar-3 cells, and Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluorophore (Fluorescein)
Cytosol and Nucleus
Equal to hLF Wt
Endocytosis and acid sphingomyelinase-dependent induction of nucleation zones (NZ)
In vitro
CCCCTHHHHTTTCCCSSSSCCC
Additional Detail Fields 1 fields
CCCCTHHHHTTTCCCSSSSCCC