TPDB

3D PDB MODEL

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TPDB ID TPDB92916

Peptide_Name

NF70 (H6-C20) NF70

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

modified_plain

Has_Modification Yes

Description A 23-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

C-terminal: Amidation N-terminal: Arachidylation

Sequence

HHHHYHHGOILLKALKALAKAIL-NH2

FASTA

>TPDB92916|Cell-penetrating Peptides|length=23
HHHHYHHGOILLKALKALAKAIL-NH2

Formula C119H189N37O24

Absent Amino Acids RNDCEQMFPSTWV

Common Amino Acids H

Mass 2522.04

PI 10.46

Basic Residues 9

Acidic Residues 0

Hydrophobic Residues 12

Net Charge +4

Boman Index 3.70

Hydrophobicity 0.113

Aliphatic Index 136.09

Half Life

Mammalian: 3.5 hour Yeast: 10 min E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 59.08

Polar Residues 1

number

4

A

0

R

0

N

0

D

0

C

0

E

0

Q

1

G

6

H

2

I

5

L

3

K

0

M

0

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 4 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 6 I: 2 L: 5 K: 3 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

23

Formula

C119H189N37O24

Mass

2522.04

PI

10.46

Net Charge

+4

Boman Index

3.70

Hydrophobicity

0.113

C_terminal_Modification

Amidation

Chirality

Modified

Ionic_State

Amphipathic

Linearity

Linear

N_terminal_Modification

Arachidylation

SMILES

N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line U87-Luc Cells

Assay & Model

In Vitro Model Balb/c mice

In Vivo Model Balb/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Non-natural residues Linear Modified Amphipathic

Source of Peptide Synthetic

Cargo Type siRNA (GFP and Luc) siRNA against blood coagulation factor VII (siFVII)

Uptake Efficiency H6-C20/siRNA shows lowest detected GFP signal

In Vitro or In Vivo In vitro and in vivo

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Keratinocytes (Kcs) And Dendritic Cells (Dcs).

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear Modified Amphipathic

Source of Peptide Synthetic

Cargo Type DyLight547-labelled microRNA-146a and siRNA

Subcellular Localization Multi-Vesicular Bodies (Mvb)

Uptake Mechanism Macropinocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSCHHHHHHSSSCCC

DSSP_Secondary_Structure

CCCSSSCHHHHHHSSSCCC