TPDB

3D PDB MODEL

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TPDB ID TPDB92715

Peptide_Name

DOX-T7-TAT-LIP DOX-TAT-LIP

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 12-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0 and Peptipedia, with an available 3D structural model.

Modification

N-terminal: Cysteine addition

Sequence

AYGRKKRRQRRR

FASTA

>TPDB92715|Cell-penetrating Peptides|length=12
AYGRKKRRQRRR

Formula C67H123N33O15

Absent Amino Acids NDCEHILMFPSTWV

Common Amino Acids R

Mass 1630.93

PI 12.71

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 2

Net Charge +8

Boman Index 6.32

Hydrophobicity -3.183

Aliphatic Index 8.33

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 91.36

Polar Residues 2

number

1

A

6

R

0

N

0

D

0

C

0

E

1

Q

1

G

0

H

0

I

0

L

2

K

0

M

0

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 1 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

12

Formula

C67H123N33O15

Mass

1630.93

PI

12.71

Net Charge

+8

Boman Index

6.32

Hydrophobicity

-3.183

C_terminal_Modification

Free

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Cysteine addition

SMILES

N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line C6 and bEnd.3 cells

Assay & Model

In Vitro Model BALB/c mice

In Vivo Model BALB/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Nanaoparticle (Liposome), Small molecule drug (Doxorubicin)

Subcellular Localization Cytosol and Nucleus

Uptake Efficiency Higher than those of DOX-LIP

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSTTHHHHCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line C6 and bEnd.3 cells

Assay & Model

In Vitro Model BALB/c mice

In Vivo Model BALB/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Nanoparticle (Liposome), Small molecule drug (Doxorubicin), protein (Transferin)

Subcellular Localization Cytosol and Nucleus

Uptake Efficiency Efficiently internalized

Uptake Mechanism Macropinocytosis

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSTTHHHHCCC

DSSP_Secondary_Structure

CCSTTHHHHCCC