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Peptide record

TPDB92684

PF14(PepFect 14) Cell-penetrating Peptides modified_plain modified
21 amino acids
Basic Information
3D PDB MODEL
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TPDB92684
PF14(PepFect 14)
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
modified_plain
Yes
A 21-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
Chemical: Nanoparticles N-terminal: Stearylation
Sequence
AGYLLGKLLOOLAAAALOOLL
Physicochemical Analysis
C82H142N18O19
RNDCEQHIMFPSTWV
L
1684.14
9.74
1
0
14
+1
-5.26
1.590
172.38
Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour
1490
88.47
1
Residue Composition
number
5
A
0
R
0
N
0
D
0
C
0
E
0
Q
2
G
0
H
0
I
8
L
1
K
0
M
0
F
0
P
0
S
0
T
0
W
1
Y
0
V
Amino Acid Distribution
A: 5 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 0 L: 8 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0
Chemical Descriptors
21
C82H142N18O19
1684.14
9.74
+1
-5.26
1.590
Free
Nanoparticles
Modified
Amphipathic
Linear
Stearylation
N[C@@H](C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)O N[C@@H](C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)O
Evidence Records 4 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Pluc 705 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear Modified Amphipathic
Synthetic
Cy5-labeled SCO
Cytoplasm and nucleus
Slight increase (up to 24%, not significant) of Cy5 signal upon complementing SCO-PF14 nanoparticles with calcium and magnesium after 24 h incubation in HeLa pLuc 705 cells
Receptor mediated endocytosis
In vitro
CTHHHHSSCCTTSCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Egfp 654

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear Modified Amphipathic
Synthetic
Cy5-labeled SCO-654
Cytoplasm and nucleus
PF14-SCO-654 nanoparticles along with 3 mM calcium or magnesium ions led to up to a 2.5-fold increase in the efficiency of the nanoparticles in HeLa EGFP 654 cells.
Receptor mediated endocytosis
In vitro
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

U87-Luc2 Reporter Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear Modified Amphipathic
Synthetic
Luciferase-suppressing siRNA
Cytoplasm and nucleus
95% decrease of luciferase expression in the case of 50 nM siRNA
Receptor mediated endocytosis
In vitro
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

U2Os Cells, Murine H2K Mdx Myoblasts And Sma Type 1 Fibroblasts

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear Modified Amphipathic
Synthetic
PMOs of two different lengths (20- and 25-mer)
Nucleus
successful delivery of splice-switching PMOs
In vitro
CTTTSCCCCHHHHHTGGGGTC
Additional Detail Fields 1 fields
CTHHHHSSCCTTSCCCC CTTTSCCCCHHHHHTGGGGTC