TPDB

3D PDB MODEL

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TPDB ID TPDB92672

Peptide_Name

PF14

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

modified_plain

Has_Modification Yes

Description A 21-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

C-terminal: Amidation Chemical: O = Ornithine N-terminal: Stearylation

Sequence

AGYLLGKLLOOLAAAALOOLL-NH2

FASTA

>TPDB92672|Cell-penetrating Peptides|length=21
AGYLLGKLLOOLAAAALOOLL-NH2

Formula C82H142N18O19

Absent Amino Acids RNDCEQHIMFPSTWV

Common Amino Acids L

Mass 1684.14

PI 9.74

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 14

Net Charge +1

Boman Index -5.26

Hydrophobicity 1.590

Aliphatic Index 172.38

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 88.47

Polar Residues 1

number

5

A

0

R

0

N

0

D

0

C

0

E

0

Q

2

G

0

H

0

I

8

L

1

K

0

M

0

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 5 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 0 L: 8 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

21

Formula

C82H142N18O19

Mass

1684.14

PI

9.74

Net Charge

+1

Boman Index

-5.26

Hydrophobicity

1.590

C_terminal_Modification

Amidation

Chemical_Modification

O = Ornithine

Chirality

Modified

Ionic_State

Cationic and amphipathic

Linearity

Linear

N_terminal_Modification

Stearylation

SMILES

N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Dm1 Myoblasts , Fibroblast

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Non-natural residues Linear Modified Cationic and amphipathic

Source of Peptide Synthetic

Cargo Type Cy5 or FAM-labelled CAG7 ASOs

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency

Uptake of ASOs was more than 10-fold higher in cells that were incubated with PF14 polyplexes compared with R9 polyplexes or naked ASOs

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Neuro2A And Ht1080

Assay & Model

In Vitro Model Neuro2a tumor bearing 4‐ to 6‐week‐old BALB/c mice

In Vivo Model Neuro2a tumor bearing 4‐ to 6‐week‐old BALB/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear Modified

Source of Peptide Synthetic

Cargo Type Plasmid DNA (pGL3)

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCSSCSCSSSCC

DSSP_Secondary_Structure

CCCSSCSCSSSCC