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Peptide record

TPDB92669

PepFect14 (PF14) PF14 Cell-penetrating Peptides modified_plain modified
21 amino acids
Basic Information
3D PDB MODEL
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TPDB92669
PepFect14 (PF14) PF14
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
modified_plain
Yes
A 21-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
C-terminal: Amidation Chemical: Nanoparticles N-terminal: Stearylation
Sequence
AGYLLGKLLOOLAAAALOOLL-NH2
Physicochemical Analysis
C82H142N18O19
RNDCEQHIMFPSTWV
L
1684.14
9.74
1
0
14
+1
-5.26
1.590
172.38
Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour
1490
88.47
1
Residue Composition
number
5
A
0
R
0
N
0
D
0
C
0
E
0
Q
2
G
0
H
0
I
8
L
1
K
0
M
0
F
0
P
0
S
0
T
0
W
1
Y
0
V
Amino Acid Distribution
A: 5 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 0 L: 8 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0
Chemical Descriptors
21
C82H142N18O19
1684.14
9.74
+1
-5.26
1.590
Amidation
Nanoparticles
Modified
Amphipathic
Linear
Stearylation
N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O
Evidence Records 4 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa And Hacat Cell Lines

Assay & Model

Six-to-ten-week-old C57BL/6J mice
Six-to-ten-week-old C57BL/6J mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Non-natural residues Linear Modified Amphipathic
Synthetic
mCherry-mRNA, luciferase-mRNA, EGFP-mRNA, and Cy5-labeled EGFP-mRNA
Cytoplasm
PF14 effectively delivers mRNA into cell lines, and the addition of CaCl2 into the nanoparticles enhances their effect in HeLa and HaCaT cells without affecting cell viability, while PS80, chloroquine, and MgCl2 had no significant effect.
HeLa cells via macropinocytosis and HaCaT cells via both macropinocytosis and clathrin-mediated endocytosis
In vitro and in vivo
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Hek293T Wt Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear Modified Amphipathic
Synthetic
Cas9 ribonucleoprotein (RNP).
The cells incubated with PF14-AB showed a large increase in mean fluorescent intensity and percentage of positive cells over the naked AB.Also, complexation of Cas9 RNP with PF14 was sufficient to achieve editing in full media (10% FBS in DMEM) at higher MRs
In vitro
CCSCBTTBCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

Normal Human Epidermal Keratinocytes

Assay & Model

8–10-week-old wild type C57BL/6 mice
8–10-week-old wild type C57BL/6 mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear Modified
Synthetic
Dy547-labelled miRNAs (MiR-146a)
When PF14 was used for the transfection, the inhibition of miR-146a targets was slightly less efficient, as only the suppression of IL-8 (2.93 ± 0.37 fold), CCL5 (4.45 ± 0.57 fold), ICAM1 (1.96 ± 0.23 fold) and CLDN1 (1.54 ± 0.17 fold) in IFN-γ-stimulated cells and IRAK1 (1.90 ± 0.24 fold) and CCL5(1.97 ± 0.27 fold) in unstimulated cells was significant
In vitro and in vivo
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

CHO Cells

Assay & Model

Male and female BALB/c mice (8-week-old)
Male and female BALB/c mice (8-week-old)

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear Modified
Synthetic
pDNA (pGL3) and p-CMV-Luc2(pLuc)
Highest gene induction in cell culture was mediated by the vectors that have the highest charge PF14-O (net charge +7) and PF6 (pH-dependent chargen +10), closely followed by PF14 (+5)
In vitro and in vivo
Additional Detail Fields 1 fields
CCSCBTTBCCC