TPDB

3D PDB MODEL

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TPDB ID TPDB92398

Peptide_Name

B4

Activity

Antibacterial Anticancer Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

Peptipedia CancerPPD 2.0 dbACP

Sequence_Type

standard

Has_Modification No

Description A 12-aa standard natural multi-activity (Antibacterial, Anticancer, and Toxicity) peptide sequence curated from Peptipedia, CancerPPD 2.0, and dbACP, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

LKKLFKKILKYL-NH2

FASTA

>TPDB92398|Antibacterial;Anticancer;Toxicity|length=12
LKKLFKKILKYL-NH2

Formula C78H135N17O14

Absent Amino Acids ARNDCEQGHMPSTWV

Common Amino Acids K

Mass 1535.04

PI 10.77

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 7

Net Charge +5

Boman Index 0.33

Hydrophobicity 0.142

Aliphatic Index 162.50

Half Life

Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 97.07

Polar Residues 1

number

0

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

4

L

5

K

0

M

1

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 4 K: 5 M: 0 F: 1 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

12

Formula

C78H135N17O14

Mass

1535.04

PI

10.77

Net Charge

+5

Boman Index

0.33

Hydrophobicity

0.142

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Analogue of BP 100

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 6.6 ± 0.5 µM

Detail Source Activity Anticancer

Activity Measurement 6.6

Target

Cancer Type Leukemia Cancer

Cell Line K-562

Tissue Affected Blood

Assay & Model

Assay MTT assay

Testing Time 48-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2017

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 5.9 ± 0.2 µM

Detail Source Activity Anticancer

Activity Measurement 5.9

Target

Cancer Type Breast Cancer

Cell Line MCF-7

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 48-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2017

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 6.3 ± 0.4 µM

Detail Source Activity Anticancer

Activity Measurement 6.3

Target

Cancer Type Breast Cancer

Cell Line MCF-7/ADM

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 48-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2017

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 7.7 ± 0.4 µM

Detail Source Activity Anticancer

Activity Measurement 7.7

Target

Cancer Type Leukemia Cancer

Cell Line K-562/ADM

Tissue Affected Blood

Assay & Model

Assay MTT assay

Testing Time 48-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2017

Evidence 5 Activity

Activity

Activity Raw

~60-80% LDH release at 8 µM

Detail Source Activity Anticancer

Target

Cancer Type Leukemia Cancer

Cell Line K-562

Tissue Affected Blood

Assay & Model

Assay LDH leakage assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2017