TPDB

3D PDB MODEL

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TPDB ID TPDB92383

Peptide_Name

LDP-NLS LDP-NLS (LN)

Activity

Antibacterial Antifungal Cell-penetrating Peptides

Activity_category_statistics

Anti-infective peptides Delivery and barrier-penetrating peptides

Database_Source

Peptipedia Antifungipept CAFPdb dbAMP CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 17-aa standard natural multi-activity (Antibacterial, Antifungal, and Cell-penetrating Peptides) peptide sequence curated from Peptipedia, Antifungipept, CAFPdb, and other sources, with an available 3D structural model.

Modification

N-terminal: Conjugated with FITC

Sequence

KWRRKLKKLRPKKKRKV

FASTA

>TPDB92383|Antibacterial;Antifungal;Cell-penetrating Peptides|length=17
KWRRKLKKLRPKKKRKV

Formula C105H194N38O18

Absent Amino Acids ANDCEQGHIMFSTY

Common Amino Acids K

Mass 2276.94

PI 12.90

Basic Residues 12

Acidic Residues 0

Hydrophobic Residues 4

Net Charge +12

Boman Index 8.71

Hydrophobicity -2.347

Aliphatic Index 62.94

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 241.55

Polar Residues 0

number

0

A

4

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

2

L

8

K

0

M

0

F

1

P

0

S

0

T

1

W

0

Y

1

V

Amino Acid Distribution

A: 0 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 2 K: 8 M: 0 F: 0 P: 1 S: 0 T: 0 W: 1 Y: 0 V: 1

Length

17

Formula

C105H194N38O18

Mass

2276.94

PI

12.90

Net Charge

+12

Boman Index

8.71

Hydrophobicity

-2.347

C_terminal_Modification

Free

Chirality

L

Linearity

Linear

N_terminal_Modification

Conjugated with FITC

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line U87Mg And T98G Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Chimeric

Uptake Efficiency More than 90% uptake efficiency in T98G cells

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCGGGGGTTGGGGCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Fusogenic

Cargo Type β-galactosidase

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency Enhanced uptake than LDP

Uptake Mechanism Macropinocytosis and clathrin mediated endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSSCCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Fusarium Solani

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L

Source of Peptide Protein derived

Cargo Type FITC dye and dextrans

Subcellular Localization Cytoplasm

Uptake Efficiency High uptake than other peptides

Uptake Mechanism Membrane damage and pore formation

In Vitro or In Vivo In vitro

DSSP_Secondary_Structure

CCCGGGGGTTGGGGCCCC CCSSCCC