TPDB

3D PDB MODEL

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TPDB ID TPDB92281

Peptide_Name

Temporin-FL Temporin-HLa

Activity

Anti-inflammatory Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Immunity and inflammation peptides Toxicity and safety peptides

Database_Source

Peptipedia CancerPPD 2.0 CAFPdb PD_Hemolysis ToxinPred 3.0 DBAASP

Sequence_Type

standard modified_plain

Has_Modification Yes

Description A 17-aa standard natural and modified multi-activity (Anti-inflammatory, Antibacterial, Anticancer, and other sources) peptide sequence curated from Peptipedia, CancerPPD 2.0, CAFPdb, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

FFPLIFGALSSILPKIL-NH2

FASTA

>TPDB92281|Anti-inflammatory;Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=17
FFPLIFGALSSILPKIL-NH2

Formula C96H150N18O20

Absent Amino Acids RNDCEQHMTWYV

Common Amino Acids L

Mass 1876.36

PI 10.10

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +1

Boman Index -4.57

Hydrophobicity 1.753

Aliphatic Index 166.47

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 2

number

1

A

0

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

3

I

4

L

1

K

0

M

3

F

2

P

2

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 3 L: 4 K: 1 M: 0 F: 3 P: 2 S: 2 T: 0 W: 0 Y: 0 V: 0

Length

17

Formula

C96H150N18O20

Mass

1876.36

PI

10.10

Net Charge

+1

Boman Index

-4.57

Hydrophobicity

1.753

C_terminal_Modification

Amidation AMD

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Hylarana latouchii

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 4.96 ± 0.94 µM

Detail Source Activity Anticancer

Activity Measurement 4.96

Target

Cancer Type Lung Cancer

Cell Line NCI-H23

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 15.18 ± 2.98 µM

Detail Source Activity Anticancer

Activity Measurement 15.18

Target

Cancer Type Lung Cancer

Cell Line H-157

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 1.64 ± 0.41 µM

Detail Source Activity Anticancer

Activity Measurement 1.64

Target

Cancer Type Lung Cancer

Cell Line H-460

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2020

Evidence 4 Activity

Target

Target Organism Cutibacterium acnes

Source & Reference

Database Source DBAASP