TPDB

3D PDB MODEL

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TPDB ID TPDB92188

Peptide_Name

Phylloseptin-PBa2 Phylloseptin-PBa3

Activity

Antibacterial Anticancer Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia CancerPPD 2.0 dbACP Antifungipept CAFPdb dbAMP Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 19-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

FLSLLPHIASGIASLVSKF-NH2

FASTA

>TPDB92188|Antibacterial;Anticancer;Antifungal;Hemolysis;Toxicity|length=19
FLSLLPHIASGIASLVSKF-NH2

Formula C96H154N22O24

Absent Amino Acids RNDCEQMTWY

Common Amino Acids LS

Mass 2000.41

PI 10.10

Basic Residues 2

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +1

Boman Index -2.46

Hydrophobicity 1.332

Aliphatic Index 148.95

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 4

number

2

A

0

R

0

N

0

D

0

C

0

E

0

Q

1

G

1

H

2

I

4

L

1

K

0

M

2

F

1

P

4

S

0

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 2 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 1 I: 2 L: 4 K: 1 M: 0 F: 2 P: 1 S: 4 T: 0 W: 0 Y: 0 V: 1

Length

19

Formula

C96H154N22O24

Mass

2000.41

PI

10.10

Net Charge

+1

Boman Index

-2.46

Hydrophobicity

1.332

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Phyllomedusa burmeisteri

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 0.29 µM

Detail Source Activity Anticancer

Activity Measurement 0.29 µM

Target

Cancer Type Breast Cancer

Cell Line MDA-MB-435S

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 72-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint Cell viability

Activity Raw

Cell viability ~ 60% at 10-5 M

Detail Source Activity Anticancer

Activity Measurement ~60 % at 10-5 M

Target

Cancer Type Brain Tumor

Cell Line U251-MG

Tissue Affected Brain

Assay & Model

Assay MTT assay

Testing Time 72-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 3 Activity

Activity

Activity Endpoint Cell viability

Activity Raw

Cell viability ~ 60% at 10-5 M

Detail Source Activity Anticancer

Activity Measurement ~60 % at 10-5 M

Target

Cancer Type Lung Cancer

Cell Line H-460

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 72-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019

Evidence 4 Activity

Activity

Activity Endpoint Cell viability

Activity Raw

Cell viability ~ 65% at 10-5 M

Detail Source Activity Anticancer

Activity Measurement ~65 % at 10-5 M

Target

Cancer Type Lung Cancer

Cell Line H-838

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 72-h

Source & Reference

Database Source CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2019