TPDB

3D PDB MODEL

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TPDB ID TPDB92104

Peptide_Name

MCoTI-II MCoTI-M6

Activity

Antibacterial Anticancer Antifungal Cell-penetrating Peptides

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 Peptipedia CAFPdb dbAMP CellPPD CellPPD-MOD CPPsite3.0 MLCPP 2.0 PerseuCPP

Sequence_Type

standard

Has_Modification No

Description A 34-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CAFPdb, and other sources, with an available 3D structural model.

Modification

N-terminal: Conjugation with Alexa Flour 488

Sequence

GGVCPKILKKCRRDSDCPGACICRGNGYCGSGSD

FASTA

>TPDB92104|Antibacterial;Anticancer;Antifungal;Cell-penetrating Peptides|length=34
GGVCPKILKKCRRDSDCPGACICRGNGYCGSGSD

Formula C138H231N47O46S6

Absent Amino Acids EQHMFTW

Common Amino Acids G

Mass 3477.00

PI 8.31

Basic Residues 6

Acidic Residues 3

Hydrophobic Residues 6

Net Charge +3

Boman Index 7.21

Hydrophobicity -0.444

Aliphatic Index 45.88

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1865

Absorbance 280nm 53.64

Polar Residues 11

number

1

A

3

R

1

N

3

D

6

C

0

E

0

Q

7

G

0

H

2

I

1

L

3

K

0

M

0

F

2

P

3

S

0

T

0

W

1

Y

1

V

Amino Acid Distribution

A: 1 R: 3 N: 1 D: 3 C: 6 E: 0 Q: 0 G: 7 H: 0 I: 2 L: 1 K: 3 M: 0 F: 0 P: 2 S: 3 T: 0 W: 0 Y: 1 V: 1

Length

34

Formula

C138H231N47O46S6

Mass

3477.00

PI

8.31

Net Charge

+3

Boman Index

7.21

Hydrophobicity

-0.444

C_terminal_Modification

Free

Chirality

L

Ionic_State

Cationic

Linearity

Cyclic

N_terminal_Modification

Conjugation with Alexa Flour 488

SMILES

NCC(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CC(=O)N)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Cyclic L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (Alexa Fluor 488)

Uptake Efficiency Higher uptake efficiency

Uptake Mechanism Macropinocytosis, endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CTTSHHHHHHHSCSCCCGGGCCCCSSSSSCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Cyclic L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (Alexa Fluor 488)

Uptake Efficiency High (compared to native MCoTI-II)

Uptake Mechanism Macropinocytosis, endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CTTSHHHHHHHSCSCCCGGGCCCCSSSSSCCCCC

DSSP_Secondary_Structure

CTTSHHHHHHHSCSCCCGGGCCCCSSSSSCCCCC