TPDB

3D PDB MODEL

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TPDB ID TPDB92074

Peptide_Name

Ple-a

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 SATPdb dbACP Antifungipept CAFPdb i2APP APD_complete HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 25-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

GWGSFFKKAAHVGKHVGKAALTHYL-NH2

FASTA

>TPDB92074|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Hemolysis;Toxicity|length=25
GWGSFFKKAAHVGKHVGKAALTHYL-NH2

Formula C129H192N36O29

Absent Amino Acids RNDCEQIMP

Common Amino Acids AGK

Mass 2711.17

PI 10.64

Basic Residues 7

Acidic Residues 0

Hydrophobic Residues 12

Net Charge +4

Boman Index 0.44

Hydrophobicity -0.068

Aliphatic Index 70.40

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 6990

Absorbance 280nm 257.82

Polar Residues 3

number

4

A

0

R

0

N

0

D

0

C

0

E

0

Q

4

G

3

H

0

I

2

L

4

K

0

M

2

F

0

P

1

S

1

T

1

W

1

Y

2

V

Amino Acid Distribution

A: 4 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 4 H: 3 I: 0 L: 2 K: 4 M: 0 F: 2 P: 0 S: 1 T: 1 W: 1 Y: 1 V: 2

Length

25

Formula

C129H192N36O29

Mass

2711.17

PI

10.64

Net Charge

+4

Boman Index

0.44

Hydrophobicity

-0.068

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

C-terminal-amidated derivative of Pleurocidin

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 11 µM

Detail Source Activity Anticancer

Activity Measurement 11 µM

Target

Cancer Type Liver Cancer

Cell Line J5

Tissue Affected Liver

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 60 µM

Detail Source Activity Anticancer

Activity Measurement 60 µM

Target

Cancer Type Liver Cancer

Cell Line Huh-7

Tissue Affected Liver

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 77.5 µM

Detail Source Activity Anticancer

Activity Measurement 77.5 µM

Target

Cancer Type Liver Cancer

Cell Line Hep3B

Tissue Affected Liver

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 42.1 µM

Detail Source Activity Anticancer

Activity Measurement 42.1 µM

Target

Cancer Type Lung Cancer

Cell Line A-549

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022

Evidence 5 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 29.8 µM

Detail Source Activity Anticancer

Activity Measurement 29.8 µM

Target

Cancer Type Stomach Cancer

Cell Line AGS

Tissue Affected Stomach

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022

Evidence 6 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 197.3 µM

Detail Source Activity Anticancer

Activity Measurement 197.3 µM

Target

Cancer Type Colon Cancer

Cell Line WiDr

Tissue Affected Colon

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022