TPDB

3D PDB MODEL

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TPDB ID TPDB92053

Peptide_Name

Piscidin 3

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 CancerPPD 2.0 iACP-DRLF AFP-GFuse Antifungipept CAFPdb i2APP AI4AVP dbAMP 3.0 DRAVP 2.0 APD_complete APD_hemolytic HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 22-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

Chemical: Cu2+ bound

Sequence

FIHHIFRGIVHAGRSIGRFLTG

FASTA

>TPDB92053|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Hemolysis;Toxicity|length=22
FIHHIFRGIVHAGRSIGRFLTG

Formula C116H179N37O25

Absent Amino Acids NDCEQKMPWY

Common Amino Acids GI

Mass 2491.93

PI 12.70

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 10

Net Charge +3

Boman Index 0.33

Hydrophobicity 0.455

Aliphatic Index 106.36

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 2

number

1

A

3

R

0

N

0

D

0

C

0

E

0

Q

4

G

3

H

4

I

1

L

0

K

0

M

3

F

0

P

1

S

1

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 1 R: 3 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 4 H: 3 I: 4 L: 1 K: 0 M: 0 F: 3 P: 0 S: 1 T: 1 W: 0 Y: 0 V: 1

Length

22

Formula

C116H179N37O25

Mass

2491.93

PI

12.70

Net Charge

+3

Boman Index

0.33

Hydrophobicity

0.455

C_terminal_Modification

Free

Chemical_Modification

Cu2+ bound

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Anticancer

Origin

Morone chrysops x Morone saxatilis

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 5.52 µM

Detail Source Activity Anticancer

Activity Measurement 5.52 µM

Target

Cancer Type Fibrosarcoma

Cell Line HT-1080

Tissue Affected Not Available

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0

Source CancerPPD2

Year 2021

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 5.25 µM

Detail Source Activity Anticancer

Activity Measurement 5.25 µM

Target

Cancer Type Breast Cancer

Cell Line MDA-MB-231

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0

Source CancerPPD2

Year 2021

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 2.99 µM

Detail Source Activity Anticancer

Activity Measurement 2.99 µM

Target

Cancer Type Lung Cancer

Cell Line A-549

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0

Source CancerPPD2

Year 2021

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 1.97 µM

Detail Source Activity Anticancer

Activity Measurement 1.97 µM

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0

Source CancerPPD2

Year 2021