TPDB

3D PDB MODEL

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TPDB ID TPDB92033

Peptide_Name

PF6(PepFect 6)

Activity

Antibacterial Anticancer Antifungal Antiparasitic Blood-Brain Barrier Cell-penetrating Peptides Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP Peptipedia Antifungipept CAFPdb dbAMP 3.0 i2APP B3Pred BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 21-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, Peptipedia, and other sources, with an available 3D structural model.

Modification

Chemical: Nanoparticles N-terminal: Stearylation

Sequence

AGYLLGKINLKALAALAKKIL

FASTA

>TPDB92033|Antibacterial;Anticancer;Antifungal;Antiparasitic;Blood-Brain Barrier;Cell-penetrating Peptides;Hemolysis;Toxicity|length=21
AGYLLGKINLKALAALAKKIL

Formula C104H184N26O24

Absent Amino Acids RDCEQHMFPSTWV

Common Amino Acids L

Mass 2182.76

PI 10.64

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 14

Net Charge +4

Boman Index -1.37

Hydrophobicity 0.933

Aliphatic Index 172.38

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 68.26

Polar Residues 2

number

5

A

0

R

1

N

0

D

0

C

0

E

0

Q

2

G

0

H

2

I

6

L

4

K

0

M

0

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 5 R: 0 N: 1 D: 0 C: 0 E: 0 Q: 0 G: 2 H: 0 I: 2 L: 6 K: 4 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

21

Formula

C104H184N26O24

Mass

2182.76

PI

10.64

Net Charge

+4

Boman Index

-1.37

Hydrophobicity

0.933

C_terminal_Modification

Free

Chemical_Modification

Nanoparticles

Chirality

L

Ionic_State

Amphipathic

Linearity

Linear

N_terminal_Modification

Stearylation

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Pluc 705 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Non-natural residues Linear L Amphipathic

Source of Peptide Synthetic

Cargo Type Cy5-labeled SCO-706

Uptake Efficiency 30-fold increase of luminescence of the HeLa pLuc 705 cells.

In Vitro or In Vivo In vitro

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line U2Os Cells, Murine H2K Mdx Myoblasts And Sma Type 1 Fibroblasts

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L Amphipathic

Source of Peptide Synthetic

Cargo Type PMOs of two different lengths (20- and 25-mer)

Subcellular Localization Nucleus

Uptake Efficiency successful delivery of splice-switching PMOs

In Vitro or In Vivo In vitro