TPDB

3D PDB MODEL

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TPDB ID TPDB91912

Peptide_Name

RT2

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP Peptipedia CancerPPD 2.0 HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 17-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from AntiBP3, dbAMP, Peptipedia, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

NGVQPKYRWWRWWRRWW-NH2

FASTA

>TPDB91912|Antibacterial;Anticancer;Hemolysis;Toxicity|length=17
NGVQPKYRWWRWWRRWW-NH2

Formula C126H164N38O21

Absent Amino Acids ADCEHILMFST

Common Amino Acids W

Mass 2546.93

PI 12.41

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +5

Boman Index -6.28

Hydrophobicity -1.965

Aliphatic Index 17.06

Half Life

Mammalian: 1.4 hour Yeast: 3 min E.coli: >10 hour

Extinction Coefficient Cystines 34490

Absorbance 280nm 1354.18

Polar Residues 3

number

0

A

4

R

1

N

0

D

0

C

0

E

1

Q

1

G

0

H

0

I

0

L

1

K

0

M

0

F

1

P

0

S

0

T

6

W

1

Y

1

V

Amino Acid Distribution

A: 0 R: 4 N: 1 D: 0 C: 0 E: 0 Q: 1 G: 1 H: 0 I: 0 L: 0 K: 1 M: 0 F: 0 P: 1 S: 0 T: 0 W: 6 Y: 1 V: 1

Length

17

Formula

C126H164N38O21

Mass

2546.93

PI

12.41

Net Charge

+5

Boman Index

-6.28

Hydrophobicity

-1.965

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial and Anticancer Antimicrobial

Origin

Crocodylus siamensis

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 28.67 ± 0.4 µM

Detail Source Activity Anticancer

Activity Measurement 28.67

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay Sulforhodamine B assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2016

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 17.28 ± 0.2 µM

Detail Source Activity Anticancer

Activity Measurement 17.28

Target

Cancer Type Cervical Cancer

Cell Line CaSki

Tissue Affected Cervix

Assay & Model

Assay Sulforhodamine B assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2016

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 90.25 µg/mL

Detail Source Activity Anticancer

Activity Measurement 90.25 µg/mL

Target

Cancer Type Colon Cancer

Cell Line HCT-116

Tissue Affected Colon

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2018

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 29.74 µM

Detail Source Activity Anticancer

Activity Measurement 29.74 µM

Target

Cancer Type Colon Cancer

Cell Line CaCo-2

Tissue Affected Colon

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2022