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Peptide record

TPDB91868

Penetratin (Pen) Antibacterial Anticancer Antifungal Antiviral Blood-Brain Barrier Cell-penetrating Peptides Toxicity standard
16 amino acids
Basic Information
3D PDB MODEL
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TPDB91868
Penetratin (Pen)
Antibacterial Anticancer Antifungal Antiviral Blood-Brain Barrier Cell-penetrating Peptides Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 iACP-DRLF pep-lab Antifungipept CAFPdb AI4AVP B3Pred BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP
standard
No
A 16-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.
Chemical: Transferrin (Tf)
Sequence
RQIKIWFQNRRMKWKK
Physicochemical Analysis
C104H168N34O20S1
ADCEGHLPSTYV
K
2246.75
12.72
7
0
6
+7
2.29
-1.731
48.75
Mammalian: 1 hour Yeast: 2 min E.coli: 2 min
11000
489.60
3
Residue Composition
number
0
A
3
R
1
N
0
D
0
C
0
E
2
Q
0
G
0
H
2
I
0
L
4
K
1
M
1
F
0
P
0
S
0
T
2
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 3 N: 1 D: 0 C: 0 E: 0 Q: 2 G: 0 H: 0 I: 2 L: 0 K: 4 M: 1 F: 1 P: 0 S: 0 T: 0 W: 2 Y: 0 V: 0
Chemical Descriptors
16
C104H168N34O20S1
2246.75
12.72
+7
2.29
-1.731
Free
Transferrin (Tf)
L
Cationic and amphiphilic
Linear
Free
N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

U87, bEnd.3 And Glial Cells

Assay & Model

Tumor bearing nude mice
Tumor bearing nude mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic and amphiphilic
Protein derived
Lissamine rhodamine-labelled liposomes doxorubicin (Dox) and Erlotinib (Erlo)
Cytoplasm and nucleus
The uptake of Dox and Elro-loaded liposomes showed more than 73% of cellular uptake in all three types of cells, which further confirmed the efficacy of Tf-Pen liposomes over single ligand or plain liposomes.Rapid and higher uptake of Tf-Pen liposomes in U87 cells compared to bEnd.3 and glial cells.
Clathrin mediated endocytosis
In vitro and in vivo
CCGGGGGGGGGGHHHHTTSCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

U87 And bEnd.3 Cells And In Vitro Brain Tumor Model

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic and amphiphilic
Protein derived
5-fluorouracil (5-FU) lissamine rhodamine-labelled liposomes
Cytoplasm and nucleus
The uptake of 5-FU loaded Tf-Pen-conjugated liposomes was found to be 66.64 ± 3.00% and 59.68 ± 4.55% in U87 and bEnd.3 cells, respectively, which was significantly higher than single ligand (47.66 ± 1.78 % and 46.28 ± 2.39 % respectively for Tf and 45.91 ± 4.62 and 43.42 ± 2.31 respectively for Pen) or plain liposomes (31.51 ± 2.92% and 28.56 ± 3.29% respectively).
Receptor mediated endocytosis
In vitro
CCCSSSTTCCCTTTTC
Additional Detail Fields 1 fields
CCGGGGGGGGGGHHHHTTSCCC CCCSSSTTCCCTTTTC