TPDB

3D PDB MODEL

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TPDB ID TPDB91846

Peptide_Name

PEN–P9–Cy3 PEN–SP9–Cy3

Activity

Antibacterial Anticancer Antifungal Antiviral Blood-Brain Barrier Cell-penetrating Peptides Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 iACP-DRLF pep-lab Antifungipept CAFPdb AI4AVP B3Pred BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP

Sequence_Type

standard

Has_Modification No

Description A 16-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Conjugated with Cyanine3(Cy3)

Sequence

RQIKIWFQNRRMKWKK

FASTA

>TPDB91846|Antibacterial;Anticancer;Antifungal;Antiviral;Blood-Brain Barrier;Cell-penetrating Peptides;Toxicity|length=16
RQIKIWFQNRRMKWKK

Formula C104H168N34O20S1

Absent Amino Acids ADCEGHLPSTYV

Common Amino Acids K

Mass 2246.75

PI 12.72

Basic Residues 7

Acidic Residues 0

Hydrophobic Residues 6

Net Charge +7

Boman Index 2.29

Hydrophobicity -1.731

Aliphatic Index 48.75

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 11000

Absorbance 280nm 489.60

Polar Residues 3

number

0

A

3

R

1

N

0

D

0

C

0

E

2

Q

0

G

0

H

2

I

0

L

4

K

1

M

1

F

0

P

0

S

0

T

2

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 3 N: 1 D: 0 C: 0 E: 0 Q: 2 G: 0 H: 0 I: 2 L: 0 K: 4 M: 1 F: 1 P: 0 S: 0 T: 0 W: 2 Y: 0 V: 0

Length

16

Formula

C104H168N34O20S1

Mass

2246.75

PI

12.72

Net Charge

+7

Boman Index

2.29

Hydrophobicity

-1.731

C_terminal_Modification

Conjugated with Cyanine3(Cy3)

Chirality

L

Linearity

Stapled

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hae Cells

Assay & Model

In Vitro Model C57BL/6J mice

In Vivo Model C57BL/6J mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Stapled L

Source of Peptide Chimeric

Cargo Type Stapled peptide SP9 and Cy3 dye

Subcellular Localization Cytoplasm

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hae Cells

Assay & Model

In Vitro Model C57BL/6J mice

In Vivo Model C57BL/6J mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Stapled L

Source of Peptide Chimeric

Cargo Type P9 peptide and Cy3 dye

Subcellular Localization Cytoplasm

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

DSSP_Secondary_Structure

CCCCCCCC CCCSSSTTCCCTTTTC