TPDB

3D PDB MODEL

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TPDB ID TPDB91799

Peptide_Name

CAME-15

Activity

Antibacterial Anticancer Antifungal Antiparasitic Cell-penetrating Peptides Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF Antifungipept CAFPdb dbAMP 3.0 CellPPD-MOD CPPsite3.0 APD_complete HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 15-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

KWKLFKKIGAVLKVL-NH2

FASTA

>TPDB91799|Antibacterial;Anticancer;Antifungal;Antiparasitic;Cell-penetrating Peptides;Hemolysis;Toxicity|length=15
KWKLFKKIGAVLKVL-NH2

Formula C89H151N21O16

Absent Amino Acids RNDCEQHMPSTY

Common Amino Acids K

Mass 1771.31

PI 11.12

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 9

Net Charge +5

Boman Index -0.04

Hydrophobicity 0.540

Aliphatic Index 149.33

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 310.50

Polar Residues 0

number

1

A

0

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

1

I

3

L

5

K

0

M

1

F

0

P

0

S

0

T

1

W

0

Y

2

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 3 K: 5 M: 0 F: 1 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 2

Length

15

Formula

C89H151N21O16

Mass

1771.31

PI

11.12

Net Charge

+5

Boman Index

-0.04

Hydrophobicity

0.540

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Anticancer

Origin

FLAK peptides

Evidence 1 Activity

Activity

Activity Endpoint LD50

Activity Raw

LD50 =45 µg/ml

Detail Source Activity Anticancer

Activity Measurement 45 µg/mL

Target

Cancer Type Breast Cancer

Cell Line MCF-7

Tissue Affected Breast

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2005

Patents US_06875744_B2

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.

Evidence 2 Activity

Activity

Activity Raw

Not Available

Detail Source Activity Anticancer

Target

Cancer Type Colon Cancer

Cell Line SW-480

Tissue Affected Colon

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2005

Patents US_06875744_B2

Evidence 3 Activity

Activity

Activity Endpoint LD50

Activity Raw

LD50 =40 µg/ml

Detail Source Activity Anticancer

Activity Measurement 40 µg/mL

Target

Cancer Type Skin Cancer

Cell Line BMKC

Tissue Affected Skin

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2005

Patents US_06875744_B2

Evidence 4 Activity

Activity

Activity Endpoint LD50

Activity Raw

LD50 =65 µg/ml

Detail Source Activity Anticancer

Activity Measurement 65 µg/mL

Target

Cancer Type Lung Cancer

Cell Line H-1299

Tissue Affected Lung

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2005

Patents US_06875744_B2

Evidence 5 Activity

Activity

Activity Endpoint LD50

Activity Raw

LD50 =40 µg/ml

Detail Source Activity Anticancer

Activity Measurement 40 µg/mL

Target

Cancer Type Prostate Cancer

Cell Line PC-3

Tissue Affected Prostate

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2005

Patents US_06875744_B2

Evidence 6 Activity

Activity

Activity Endpoint LD50

Activity Raw

LD50 =30 µg/ml

Detail Source Activity Anticancer

Activity Measurement 30 µg/mL

Target

Cancer Type Cervical Cancer

Cell Line HeLa

Tissue Affected Cervix

Assay & Model

Assay MTT/MTS assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2005

Patents US_06875744_B2