TPDB

3D PDB MODEL

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TPDB ID TPDB91783

Peptide_Name

N-3L N-4L

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP dbAMP 3.0 iACP-DRLF HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 15-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

WFKKIPKFLHLLKKF-NH2

FASTA

>TPDB91783|Antibacterial;Anticancer;Hemolysis;Toxicity|length=15
WFKKIPKFLHLLKKF-NH2

Formula C103H157N23O16

Absent Amino Acids ARNDCEQGMSTYV

Common Amino Acids K

Mass 1973.52

PI 11.12

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +5

Boman Index -0.87

Hydrophobicity -0.060

Aliphatic Index 104.00

Half Life

Mammalian: 2.8 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 278.69

Polar Residues 0

number

0

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

1

H

1

I

3

L

5

K

0

M

3

F

1

P

0

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 1 I: 1 L: 3 K: 5 M: 0 F: 3 P: 1 S: 0 T: 0 W: 1 Y: 0 V: 0

Length

15

Formula

C103H157N23O16

Mass

1973.52

PI

11.12

Net Charge

+5

Boman Index

-0.87

Hydrophobicity

-0.060

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antibacterial

Origin

Ceropin A

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50=5 µM

Detail Source Activity Anticancer

Activity Measurement 5 µM

Target

Cancer Type Breast Cancer

Cell Line MDA-MB-361

Tissue Affected Breast

Assay & Model

Assay MTT/MTS assay

Testing Time 48-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2001

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50=6 µM

Detail Source Activity Anticancer

Activity Measurement 6 µM

Target

Cancer Type Blood Cancer

Cell Line Jurkat

Tissue Affected Blood

Assay & Model

Assay MTT/MTS assay

Testing Time 48-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2001

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50=3 µM

Detail Source Activity Anticancer

Activity Measurement 3 µM

Target

Cancer Type Leukemia Cancer

Cell Line K-562

Tissue Affected Blood

Assay & Model

Assay MTT/MTS assay

Testing Time 48-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2001