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Peptide record

TPDB91718

pTat TAT TAT(48–60) Antibacterial Anticancer Cell-penetrating Peptides Toxicity Tumor-homing Peptides standard
13 amino acids
Basic Information
3D PDB MODEL
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TPDB91718
pTat TAT TAT(48–60)
Antibacterial Anticancer Cell-penetrating Peptides Toxicity Tumor-homing Peptides
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP TumorHoPe2.0
standard
No
A 13-aa standard natural multi-activity (Antibacterial, Anticancer, Cell-penetrating Peptides, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CellPPD, and other sources, with an available 3D structural model.
C-terminal: Amidation
Sequence
GRKKRRQRRRPPQ-NH2
Physicochemical Analysis
C70H131N35O16
ANDCEHILMFSTWYV
R
1719.04
13.10
8
0
0
+8
8.91
-3.492
0.00
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
2
Residue Composition
number
0
A
6
R
0
N
0
D
0
C
0
E
2
Q
1
G
0
H
0
I
0
L
2
K
0
M
0
F
2
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 2 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 2 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
13
C70H131N35O16
1719.04
13.10
+8
8.91
-3.492
Amidation
L
Cationic
Linear Tetramer
Free
NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)O N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)O N[C@@H](C(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)O
Evidence Records 7 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Swiss 3T3 cells and bovine spermatozoa

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Protein derived
Fluorophore (TAMRA)
Direct Translocation
In vitro
CCCCCCCCCCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Rbl-2H3 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
TAMRA dye
Lysosomes
Translocation efficacies (mean ± S.E.M. n = 6) is 4.1 ± 1.2 for TAMRA–Tat
In vitro
CCCCSCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

Rbl-2H3 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Avidin–TXR
Lysosomes
Translocation efficacies (mean ± S.E.M. n = 6) is 4.9 ± 0.8 for Btn–Tat–Avidin–TXR
In vitro
CCCCSCCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

CHO-K1 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
FITC labelled
Internalization efficacy approximately 5- to 10-fold lower compared to PACAP38
In vitro
CCSCCSCCTTTHHHIIIIIHHHTTTTC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

Giant Plasma Membrane Vesicles (Gpmv)Of Mb-231 And Rbl-2H3 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Fluor 633 (AF633) dye
In vitro
CCCTTCCSCGGGGGGHHHHTTC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

Sk-Br-3, Jimt-1, A431, Jurkat-Cells

Assay & Model

Athymic BALB/c nude mice xenografted A431tumors
Athymic BALB/c nude mice xenografted A431tumors

Source & Reference

CPPsite3.0
CPPsite3
EP2928502B1

Other

Natural residues Tetramer L Cationic
Protein derived
Anti-EGFR mAb matuzumab (Mat) , mAb trastuzumab (Her) and ADC adotrastuzumab-emtansine (Kad)
Vesicles
Highest values as 46% for Mat-tTAT
Endocytosis
In vitro and in vivo
CCCSSSTTCCCTTTTC
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Assay & Model

Balb/c female mice (H-2d haplotype) of 8 to 12 weeks
Balb/c female mice (H-2d haplotype) of 8 to 12 weeks

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Recombinant hepatitis B surface protein (HBsAg)
Cytoplasm and Endosomes
Endocytosis
In vivo
CCSCC
Additional Detail Fields 1 fields
CCCCCCCCCCCCC CCCCSCCC CCSCCSCCTTTHHHIIIIIHHHTTTTC CCCTTCCSCGGGGGGHHHHTTC CCCSSSTTCCCTTTTC CCSCC