TPDB

3D PDB MODEL

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TPDB ID TPDB91705

Peptide_Name

TatP59W

Activity

Antibacterial Cell-penetrating Peptides Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 Peptipedia CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Cell-penetrating Peptides, and Toxicity) peptide sequence curated from AntiBP3, Peptipedia, CellPPD, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation N-terminal: Conjugation with fluorescein

Sequence

GRKKRRQRRRPWQ-NH2

FASTA

>TPDB91705|Antibacterial;Cell-penetrating Peptides;Toxicity|length=13
GRKKRRQRRRPWQ-NH2

Formula C76H134N36O16

Absent Amino Acids ANDCEHILMFSTYV

Common Amino Acids R

Mass 1808.13

PI 13.10

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 1

Net Charge +8

Boman Index 6.61

Hydrophobicity -3.438

Aliphatic Index 0.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 304.18

Polar Residues 2

number

0

A

6

R

0

N

0

D

0

C

0

E

2

Q

1

G

0

H

0

I

0

L

2

K

0

M

0

F

1

P

0

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 2 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 1 S: 0 T: 0 W: 1 Y: 0 V: 0

Length

13

Formula

C76H134N36O16

Mass

1808.13

PI

13.10

Net Charge

+8

Boman Index

6.61

Hydrophobicity

-3.438

C_terminal_Modification

Amidation

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Conjugation with fluorescein

SMILES

NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCC(=O)N)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line PC-12 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Fluorophore (fluorescein)

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency Comparable to that of (pAntp) (43-58)

Uptake Mechanism Endocytic pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCCCSCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line V79 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type Fluorophore (fluorescein)

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency Comparable to that of (pAntp) (43-58)

Uptake Mechanism Non-endocytic mechanism

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCCCSCC

DSSP_Secondary_Structure

CCCCCCCCCCSCC