TPDB

3D PDB MODEL

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TPDB ID TPDB91702

Peptide_Name

temporin-PEa

Activity

Antibacterial Anticancer Antifungal Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 Antifungipept CAFPdb

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

FLYIVAKLLSGLL-NH2

FASTA

>TPDB91702|Antibacterial;Anticancer;Antifungal;Toxicity|length=13
FLYIVAKLLSGLL-NH2

Formula C73H120N14O16

Absent Amino Acids RNDCEQHMPTW

Common Amino Acids L

Mass 1449.84

PI 9.74

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 10

Net Charge +1

Boman Index -4.15

Hydrophobicity 1.992

Aliphatic Index 210.00

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 102.77

Polar Residues 2

number

1

A

0

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

1

I

5

L

1

K

0

M

1

F

0

P

1

S

0

T

0

W

1

Y

1

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 5 K: 1 M: 0 F: 1 P: 0 S: 1 T: 0 W: 0 Y: 1 V: 1

Length

13

Formula

C73H120N14O16

Mass

1449.84

PI

9.74

Net Charge

+1

Boman Index

-4.15

Hydrophobicity

1.992

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial and Anticancer

Origin

Synthetic analog of Temporin-PE

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 3.398 µM

Detail Source Activity Anticancer

Activity Measurement 3.398 µM

Target

Cancer Type Lung Cancer

Cell Line NCI-H-157

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2018

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 3.520 µM

Detail Source Activity Anticancer

Activity Measurement 3.520 µM

Target

Cancer Type Brain Tumor

Cell Line U251-MG

Tissue Affected Brain

Assay & Model

Assay MTT assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2018

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 27.62 µM

Detail Source Activity Anticancer

Activity Measurement 27.62 µM

Target

Cancer Type Prostate Cancer

Cell Line PC-3

Tissue Affected Prostate

Assay & Model

Assay MTT assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2018

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 9.864 µM

Detail Source Activity Anticancer

Activity Measurement 9.864 µM

Target

Cancer Type Skin Cancer

Cell Line MDA-MB-435S

Tissue Affected Skin

Assay & Model

Assay MTT assay

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0

Source CancerPPD2

Year 2018