TPDB

3D PDB MODEL

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TPDB ID TPDB91635

Peptide_Name

ASA-Tat

Activity

Antibacterial Anticancer Antifungal Blood-Brain Barrier Cell-penetrating Peptides Toxicity Tumor-homing Peptides

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 Peptipedia CancerPPD 2.0 SATPdb dbACP CAFPdb B3Pred BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 11-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Modification

N-terminal: Conjugated with ASA drug

Sequence

YGRKKRRQRRR

FASTA

>TPDB91635|Antibacterial;Anticancer;Antifungal;Blood-Brain Barrier;Cell-penetrating Peptides;Toxicity;Tumor-homing Peptides|length=11
YGRKKRRQRRR

Formula C64H118N32O14

Absent Amino Acids ANDCEHILMFPSTWV

Common Amino Acids R

Mass 1559.85

PI 12.71

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 1

Net Charge +8

Boman Index 6.49

Hydrophobicity -3.636

Aliphatic Index 0.00

Half Life

Mammalian: 2.8 hour Yeast: 10 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 95.52

Polar Residues 2

number

0

A

6

R

0

N

0

D

0

C

0

E

1

Q

1

G

0

H

0

I

0

L

2

K

0

M

0

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 0 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

11

Formula

C64H118N32O14

Mass

1559.85

PI

12.71

Net Charge

+8

Boman Index

6.49

Hydrophobicity

-3.636

Conformation

Linear

N_terminal_Modification

Conjugated with ASA drug

Peptide_Length

Similarity

Peptide_Sequence_3Letter

TyrGlyArgLysLysArgArgGlnArgArgArg

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line CHO cell line

Assay & Model

In Vitro Concentration 2.5 microgram/ ml

In Vitro Method cellular uptake assay

In Vitro Result 12.6 % cellular uptake

Animal Model Origin/Source

Physical Condition Lysosomal disorder

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transendocytosis

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line bEND cell line

Assay & Model

In Vitro Concentration 2.5 microgram/ ml

In Vitro Method cellular uptake assay

In Vitro Result 16.6 % cellular uptake

Animal Model Origin/Source

Physical Condition Lysosomal disorder

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transendocytosis

Evidence 3 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line PBCECs cell line

Assay & Model

In Vitro Concentration 0.02% cells were added

In Vitro Method Basolateral screening assay

In Vitro Result 100% transfer

Animal Model Origin/Source

Physical Condition Lysosomal disorder

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Basolateral