TPDB

3D PDB MODEL

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TPDB ID TPDB91443

Peptide_Name

Temporin-SHf

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 dbACP CAFPdb dbAMP 3.0 APD_complete HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 8-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

FFFLSRIF-NH2

FASTA

>TPDB91443|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Hemolysis;Toxicity|length=8
FFFLSRIF-NH2

Formula C57H77N11O10

Absent Amino Acids ANDCEQGHKMPTWYV

Common Amino Acids F

Mass 1076.31

PI 11.05

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 6

Net Charge +1

Boman Index -4.45

Hydrophobicity 1.775

Aliphatic Index 97.50

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

0

A

1

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

1

L

0

K

0

M

4

F

0

P

1

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 1 K: 0 M: 0 F: 4 P: 0 S: 1 T: 0 W: 0 Y: 0 V: 0

Length

8

Formula

C57H77N11O10

Mass

1076.31

PI

11.05

Net Charge

+1

Boman Index

-4.45

Hydrophobicity

1.775

C_terminal_Modification

Amidation

Chirality

L

Linear_or_Cyclic

Linear

N_terminal_Modification

Free

Nature

Antimicrobial

Origin

Pelophylax saharica

Evidence 1 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 24.03 ± 0.945 µM

Detail Source Activity Anticancer

Activity Measurement 24.03

Target

Cancer Type Lung Cancer

Cell Line A-549

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2024

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 32.76 ± 1.528 µM

Detail Source Activity Anticancer

Activity Measurement 32.76

Target

Cancer Type Breast Cancer

Cell Line MCF-7

Tissue Affected Breast

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2024

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 36.67 ± 0.729 µM

Detail Source Activity Anticancer

Activity Measurement 36.67

Target

Cancer Type Prostate Cancer

Cell Line PC-3

Tissue Affected Prostate

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2024

Evidence 4 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 32.64 ± 1.350 µM

Detail Source Activity Anticancer

Activity Measurement 32.64

Target

Cancer Type Liver Cancer

Cell Line HepG-2

Tissue Affected Liver

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source dbAMP 3.0 CancerPPD 2.0 dbACP

Source CancerPPD2

Year 2024