TPDB

3D PDB MODEL

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TPDB ID TPDB91439

Peptide_Name

GC/R8-Lip SA-R8 StA-R8 Stearyl-R8 STR-Arg8

Activity

Antibacterial Antiviral Blood-Brain Barrier Cell-penetrating Peptides Tumor-homing Peptides

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 8-aa standard natural multi-activity (Antibacterial, Antiviral, Blood-Brain Barrier, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

N-terminal: Stearylation

Sequence

RRRRRRRR

FASTA

>TPDB91439|Antibacterial;Antiviral;Blood-Brain Barrier;Cell-penetrating Peptides;Tumor-homing Peptides|length=8
RRRRRRRR

Formula C48H98N32O9

Absent Amino Acids ANDCEQGHILKMFPSTWYV

Common Amino Acids R

Mass 1267.52

PI 13.25

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +8

Boman Index 6.48

Hydrophobicity -4.500

Aliphatic Index 0.00

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

0

A

8

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

0

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

8

Formula

C48H98N32O9

Mass

1267.52

PI

13.25

Net Charge

+8

Boman Index

6.48

Hydrophobicity

-4.500

C_terminal_Modification

Free

Chirality

L

Ionic_State

Cationic Amphipathic

Linearity

Linear

N_terminal_Modification

Stearylation

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](Cc1ccccc1)C(=O)/N=C/C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line JAWSII cells

Assay & Model

In Vitro Model C57BL/6 (H-2b) female mice

In Vivo Model C57BL/6 (H-2b) female mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Protein [GC (-galactosylceramide), ovalbumin]

Uptake Efficiency Highly efficient.

Uptake Mechanism Macropinocytosis

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cell

Assay & Model

In Vitro Model Male Guangxi Bama mini-pigs (10±0.5 kg, 5 to 7 months old)

In Vivo Model Male Guangxi Bama mini-pigs (10±0.5 kg, 5 to 7 months old)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Coumarin-6 labelled TDCS modified PLGA nanoparticles

Uptake Efficiency TDCS-R8 NPs higher than those for TDCS NPs and for PVA NPs but lower than TDCS-Tat NPs

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCTTSSCCCCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hek293T And Ct26.Cl25 Cell Lines

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Synthetic

Cargo Type 5-methoxyuridine-modified mCherry mRNA, Enhanced green fluorescence protein (EGFP) mRNA and Ovalbumin (OVA) mRNA

Subcellular Localization Cytoplasm

Uptake Efficiency Compared with Arg8/mRNA, the uptake rate of STR-Arg8/mRNA complexes increased approximately 7.5-fold.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSSSSSSGGGSCC

Evidence 4 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hep G2 And A549 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Cy3 and FAM-labeled siRNAs

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency StA-R8 complexes showed the highest fluorescence intensity and 40~50 times higher than R8 complexes.

Uptake Mechanism Clathrin-mediated and actin-dependent pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 5 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line A549

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type PTX and Coumarin 6- labelled nanostructured lipid carrier (NLC)

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency Internalization of R-NLC was around 4 times higher than NLC.

Uptake Mechanism Clathrin- and Caveolae-dependent endocytosis and macropinocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSGGGGSCCCCC

DSSP_Secondary_Structure

CCCCCCCC CCTTSSCCCCC CCSSSSSSGGGSCC CCSGGGGSCCCCC