TPDB

3D PDB MODEL

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TPDB ID TPDB91383

Peptide_Name

19F-labeled octaarginine (19F-R8) F(SG)4R8 G(SG)4R8 R8

Activity

Antibacterial Antiviral Blood-Brain Barrier Cell-penetrating Peptides Tumor-homing Peptides

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 8-aa standard natural multi-activity (Antibacterial, Antiviral, Blood-Brain Barrier, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation N-terminal: Acetylation

Sequence

Ac-RRRRRRRR-NH2

FASTA

>TPDB91383|Antibacterial;Antiviral;Blood-Brain Barrier;Cell-penetrating Peptides;Tumor-homing Peptides|length=8
Ac-RRRRRRRR-NH2

Formula C48H98N32O9

Absent Amino Acids ANDCEQGHILKMFPSTWYV

Common Amino Acids R

Mass 1267.52

PI 13.25

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +8

Boman Index 6.48

Hydrophobicity -4.500

Aliphatic Index 0.00

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

0

A

8

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

0

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

8

Formula

C48H98N32O9

Mass

1267.52

PI

13.25

Net Charge

+8

Boman Index

6.48

Hydrophobicity

-4.500

C_terminal_Modification

Amidation

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Acetylation

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line CHO-K1 cells, HeLa

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Nucleic acid (GAPDH siRNA)

Subcellular Localization Cytoplasm

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Glioblastoma U251

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Nucleic acid (siRNA and miRNA)

Subcellular Localization Cytoplasm

Uptake Efficiency More than 50% of cells had anti-miRNA/R8 complexes and 68% of anti-miRNA escapes the endosome/lysosome.

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, KG1a cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (Alexa488), Protein (bovine serum albumin) and Nanoparticle (quantum dots)

Uptake Efficiency Higher uptake efficiency for quantum dots

Uptake Mechanism Endocytic pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 4 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, KG1a cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (Alexa488), Protein (bovine serum albumin) and Nanoparticle (quantum dots)

Uptake Efficiency Higher uptake efficiency for quantum dots

Uptake Mechanism Endocytic pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 5 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hl60 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type 4-trifluoromethyl-L-phenylalanine (4CF3-Phe) at N terminus

Subcellular Localization Cytosol

Uptake Mechanism Direct translocation via binding to GAGs

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 6 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line CHO-K1 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type FAM via glycylglycine linker at N terminus

Uptake Efficiency Arginine peptides (FAM-R8 and FAM-Rev) significantly penetrate cell membranes

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCCCCCC

DSSP_Secondary_Structure

CCCCCCCC CCCCCCCCCCCCC