Peptide record

TPDB91375

27R (GR8) Br-Vind-(D)-Trp-Arg8 Br-Vind-(L)-Trp-Arg8 Br-Vind-Arg8 Compound 15 (octaarginine (R8)) R8 Antibacterial Antiviral Blood-Brain Barrier Cell-penetrating Peptides Tumor-homing Peptides standard

8 amino acids

3D PDB MODEL

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TPDB ID TPDB91375

Peptide_Name

27R (GR8) Br-Vind-(D)-Trp-Arg8 Br-Vind-(L)-Trp-Arg8 Br-Vind-Arg8 Compound 15 (octaarginine (R8)) R8

Activity

Antibacterial Antiviral Blood-Brain Barrier Cell-penetrating Peptides Tumor-homing Peptides

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 8-aa standard natural multi-activity (Antibacterial, Antiviral, Blood-Brain Barrier, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

RRRRRRRR-NH2

FASTA

>TPDB91375|Antibacterial;Antiviral;Blood-Brain Barrier;Cell-penetrating Peptides;Tumor-homing Peptides|length=8
RRRRRRRR-NH2

Formula C48H98N32O9

Absent Amino Acids ANDCEQGHILKMFPSTWYV

Common Amino Acids R

Mass 1267.52

PI 13.25

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +8

Boman Index 6.48

Hydrophobicity -4.500

Aliphatic Index 0.00

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

Formula

C48H98N32O9

Mass

1267.52

13.25

Net Charge

Boman Index

6.48

Hydrophobicity

-4.500

C_terminal_Modification

Amidation

Chirality

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CS)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Rpmi8226 And Kms-18

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type RID-F derivatives

Uptake Efficiency

Free RID-F did not inhibit CT-L, PGPH, and T-L activities of the intracellular proteasome in KMS-11 cells, whereas R8G-RID-F and RID-F-GR8 significantly inhibited all three activities to a similar extent.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cell Line

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line MCF-7, MDA-MB-231, Hl-60 , C26 And P388 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Br-vindoline conjugated

In Vitro or In Vivo In vitro

Evidence 4 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line MCF-7, MDA-MB-231 And Hl-60 Cells

Assay & Model

In Vitro Model P388 and C26 cells bearing BDF1 or BALB/c mice models

In Vivo Model P388 and C26 cells bearing BDF1 or BALB/c mice models

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Br-vindoline containing (L)-Trp-OMe moitey

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCC

Evidence 5 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line MCF-7, MDA-MB-231 And Hl-60 Cells

Assay & Model

In Vitro Model P388 and C26 cells bearing BDF1 or BALB/c mice models

In Vivo Model P388 and C26 cells bearing BDF1 or BALB/c mice models

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Br-vindoline containing (D)-Trp-OMe moitey

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCCCC

Evidence 6 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorescein via Ahx linker

Uptake Efficiency uptake is low

In Vitro or In Vivo In vitro

Evidence 7 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Mda, HeLa, A549 Cells And Ht-29 Human Cell Lines

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type RhB (rhodamine B with piperazine linker) at N terminus

Uptake Efficiency 0.6 microMolar uptake in HeLa cells relative to TAT 1 μM

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCGGGTSCC

Evidence 8 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type FITC dye

Subcellular Localization Cytosol

Uptake Efficiency Improved interactions between the EpN18 peptide and the cell membrane

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCTBTTTBCSSCCCCCCCC

DSSP_Secondary_Structure

CCCCCCCC CCCCCC CCGGGTSCC CCCCTBTTTBCSSCCCCCCCC