TPDB

3D PDB MODEL

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TPDB ID TPDB91309

Peptide_Name

Nisin Z Nisin ZP

Activity

Antibacterial Anticancer Antifungal Antiparasitic

Activity_category_statistics

Anti-infective peptides Cancer-related peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF Antifungipept

Sequence_Type

standard

Has_Modification No

Description A 34-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Modification

C-terminal: Cyclized N-terminal: Cyclized

Sequence

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

FASTA

>TPDB91309|Antibacterial;Anticancer;Antifungal;Antiparasitic|length=34
ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

Formula C141H245N41O46S7

Absent Amino Acids RDEQFWY

Common Amino Acids CT

Mass 3475.16

PI 8.48

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 10

Net Charge +3

Boman Index 2.49

Hydrophobicity 0.406

Aliphatic Index 71.76

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 250

Absorbance 280nm 7.19

Polar Residues 16

number

2

A

0

R

2

N

0

D

5

C

0

E

0

Q

3

G

1

H

3

I

2

L

3

K

2

M

0

F

1

P

4

S

5

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 2 R: 0 N: 2 D: 0 C: 5 E: 0 Q: 0 G: 3 H: 1 I: 3 L: 2 K: 3 M: 2 F: 0 P: 1 S: 4 T: 5 W: 0 Y: 0 V: 1

Length

34

Formula

C141H245N41O46S7

Mass

3475.16

PI

8.48

Net Charge

+3

Boman Index

2.49

Hydrophobicity

0.406

C_terminal_Modification

Cyclized

Chirality

L

Linear_or_Cyclic

Cyclic

N_terminal_Modification

Cyclized

Nature

Antimicrobial

Origin

Lactococcus lactis

Evidence 1 Activity

Activity

Activity Raw

79.22% cytotoxicity at 1000 µg/mL

Detail Source Activity Anticancer

Target

Cancer Type Colon Cancer

Cell Line SW-480

Tissue Affected Colon

Assay & Model

Assay MTT assay

Testing Time 24-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2017

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 2 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 132.4 ± 6.4 µM

Detail Source Activity Anticancer

Activity Measurement 132.4

Target

Cancer Type Lung Cancer

Cell Line A-549

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 48-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022

Evidence 3 Activity

Activity

Activity Endpoint IC50

Activity Raw

IC50 = 137.3 ± 9.1 µM

Detail Source Activity Anticancer

Activity Measurement 137.3

Target

Cancer Type Lung Cancer

Cell Line H-1299

Tissue Affected Lung

Assay & Model

Assay MTT assay

Testing Time 48-h

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2022