TPDB

TPDB ID TPDB77551

Peptide_Name

Agouti-related prote

Activity

Blood-Brain Barrier

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

Peptipedia

Sequence_Type

modified_plain

Has_Modification Yes

Description A 54-aa modified blood-brain barrier peptide sequence curated from Peptipedia, without an available 3D structural model.

Modification

C-terminal: Amidation C-terminal: Chemical modification Chemical: Cys5-Cys20, Cys12-Cys26, Cys19-Cys37, Cys23-Cys47, N-terminal: C-terminal modification

Sequence

SSRRCVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTAMNPCSRT-NH2

FASTA

>TPDB77551|Blood-Brain Barrier|length=54
SSRRCVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTAMNPCSRT-NH2

Formula C245H384N80O71S11

Absent Amino Acids IW

Common Amino Acids C

Mass 5938.90

PI 8.32

Basic Residues 9

Acidic Residues 2

Hydrophobic Residues 14

Net Charge +5

Boman Index 5.80

Hydrophobicity -0.274

Aliphatic Index 37.96

Half Life

Mammalian: 1.9 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 3605

Absorbance 280nm 60.70

Polar Residues 24

number

3

A

6

R

3

N

1

D

10

C

1

E

2

Q

2

G

2

H

0

I

3

L

1

K

1

M

3

F

3

P

4

S

3

T

0

W

2

Y

2

V

Amino Acid Distribution

A: 3 R: 6 N: 3 D: 1 C: 10 E: 1 Q: 2 G: 2 H: 2 I: 0 L: 3 K: 1 M: 1 F: 3 P: 3 S: 4 T: 3 W: 0 Y: 2 V: 2

Length

54

Formula

C245H384N80O71S11

Mass

5938.90

PI

8.32

Net Charge

+5

Boman Index

5.80

Hydrophobicity

-0.274

C_terminal_Modification

Chemical modification

Chemical_Modification

Cys5-Cys20, Cys12-Cys26, Cys19-Cys37, Cys23-Cys47,

Conformation

Linear

N_terminal_Modification

C-terminal modification

Peptide_Length

Similarity

Peptide_Sequence_3Letter

Ser-Ser-Arg-Arg-Cys-Val-Arg-Leu-His-Glu-Ser-Cys-Leu-Gly-Gln-Gln-Val-Pro-Cys-Cys-Asp-Pro-Cys-Ala-Thr-Cys-Tyr-Cys-Arg-Phe-Phe-Asn-Ala-Phe-Cys-Tyr-Cys-Arg-Lys-Leu-Gly-Thr-Ala-Met-Asn-Pro-Cys-Ser-Arg-Thr-NH2

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.03136 ml/g

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response Capillary depletion (brain/serum)

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.00455 ml/g

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response Capillary depletion (capillary/serum)

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 3 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.02682 ml/g

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response Capillary depletion (parenchyma/serum)

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 4 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 83%

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response % parenchyma

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 5 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 81 min

Assay & Model

In Vitro Method HPLC

Animal Model Origin/Source

Response half life, serum

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 6 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 289 min

Assay & Model

In Vitro Method HPLC

Animal Model Origin/Source

Response half life, brain

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 7 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.000057 mL/(g x min)

Assay & Model

Animal Model Origin/Source

Response Unidirectional influx rate constant

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

In Vivo Mode of Delivery Intravenous

Transport Type Permeability

Label Direct iodination

In_Vivo_Mode_of_Delivery

Intravenous