TPDB

TPDB ID TPDB77509

Peptide_Name

Api88

Activity

Blood-Brain Barrier

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

Peptipedia

Sequence_Type

modified_plain

Has_Modification Yes

Description A 27-aa modified blood-brain barrier peptide sequence curated from Peptipedia, without an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

Guan-ONNRPVYIPRPRPPHPRL-NH2

FASTA

>TPDB77509|Blood-Brain Barrier|length=27
Guan-ONNRPVYIPRPRPPHPRL-NH2

Formula C113H178N42O28

Absent Amino Acids DCEQKMFSTW

Common Amino Acids P

Mass 2572.92

PI 12.40

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 5

Net Charge +4

Boman Index 7.64

Hydrophobicity -1.311

Aliphatic Index 43.33

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 57.91

Polar Residues 5

number

1

A

4

R

4

N

0

D

0

C

0

E

0

Q

1

G

2

H

1

I

1

L

0

K

0

M

0

F

6

P

0

S

0

T

0

W

1

Y

1

V

Amino Acid Distribution

A: 1 R: 4 N: 4 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 2 I: 1 L: 1 K: 0 M: 0 F: 0 P: 6 S: 0 T: 0 W: 0 Y: 1 V: 1

Length

27

Formula

C113H178N42O28

Mass

2572.92

PI

12.40

Net Charge

+4

Boman Index

7.64

Hydrophobicity

-1.311

Conformation

Linear

Peptide_Length

Similarity

Peptide_Sequence_3Letter

Guan-Orn-Asn-Asn-Arg-Pro-Val-Tyr-Ile-Pro-Arg-Pro-Arg-Pro-Pro-His-Pro-Arg-Leu-NH2

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 11.36 min

Assay & Model

In Vitro Method Efflux study ICU

Animal Model Origin/Source

Response half life, brain

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Transport Type Permeability

Label Direct iodination

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.061 min-1

Assay & Model

In Vitro Method Efflux study ICU

Animal Model Origin/Source

Response Brain elemination rate constant

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Transport Type Permeability

Label Direct iodination

Evidence 3 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 22.50%

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response % capillary

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 4 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 77.50%

Assay & Model

In Vitro Method Capillary depletion

Animal Model Origin/Source

Response % parenchyma

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

Label Direct iodination

Evidence 5 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.00037 mL/(g x min)

Assay & Model

Animal Model Origin/Source

Response Unidirectional influx rate constant

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

In Vivo Mode of Delivery Intravenous

Transport Type Permeability

Label Direct iodination

Evidence 6 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Activity Result 0.02606 ml/g

Assay & Model

Animal Model Origin/Source

Response Initial disrobution volume

Source & Reference

Database Source NA

Source B3Pdb

Other

Source Origin Chemically synthesized

In Vivo Mode of Delivery Intravenous

Transport Type Permeability

Label Direct iodination

In_Vivo_Mode_of_Delivery

Intravenous