TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB72736

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 46-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

ALPCRCEGKTEYGDKWIFHGGCPNDYGYNDRCFMKPGSVCCYPKYE

FASTA

>TPDB72736|Neurotoxin|length=46
ALPCRCEGKTEYGDKWIFHGGCPNDYGYNDRCFMKPGSVCCYPKYE

Formula C233H335N61O68S7

Absent Amino Acids Q

Common Amino Acids CG

Mass 5303.01

PI 6.76

Basic Residues 7

Acidic Residues 6

Hydrophobic Residues 13

Net Charge -0

Boman Index 7.81

Hydrophobicity -0.798

Aliphatic Index 25.43

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 13325

Absorbance 280nm 251.27

Polar Residues 15

number

1

A

2

R

2

N

3

D

6

C

3

E

0

Q

6

G

1

H

1

I

1

L

4

K

1

M

2

F

4

P

1

S

1

T

1

W

5

Y

1

V

Amino Acid Distribution

A: 1 R: 2 N: 2 D: 3 C: 6 E: 3 Q: 0 G: 6 H: 1 I: 1 L: 1 K: 4 M: 1 F: 2 P: 4 S: 1 T: 1 W: 1 Y: 5 V: 1

Length

46

Formula

C233H335N61O68S7

Mass

5303.01

PI

6.76

Net Charge

-0

Boman Index

7.81

Hydrophobicity

-0.798

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Neurotoxin that induces paralysis (but not death) to U.thayeri crabs. Partially and reversibly inhibits glutamate-evoked peak currents (IC(50)=4.7 uM) but not voltage-gated potassium channel currents in cultured isolated neurons from the land snail H.aspersa. Weakly inhibits voltage-gated potassium peak currents (IC(50)=6.4 uM) and steady-state currents (IC(50)=8.2 uM) in rat dorsal root ganglion (DRG) neurons. Weakly inhibits voltage-gated sodium currents in rat DRG neurons (IC(50)=0.9 uM). {ECO:0000269|PubMed:29414882}.

Target

Target or Channel voltage-gated sodium channel (Nav) voltage-gated potassium channel (Kv)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Phymanthus crucifer (Red beaded anemone)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Neurotoxin that induces paralysis (but not death) to U.thayeri crabs. Partially and reversibly inhibits glutamate-evoked peak currents (IC(50)=4.7 uM) but not voltage-gated potassium channel currents in cultured isolated neurons from the land snail H.aspersa.

Protein Name Toxin PhcrTx2

Venom Taxon Group sea anemone

Protein Family Sea anemone type 3 (BDS) potassium channel toxin family

Protein Existence Evidence at protein level

Keywords Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin

Keywords

Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Potassium channel impairing toxin Secreted Toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Sea anemone type 3 (BDS) potassium channel toxin family

Protein_Name

Toxin PhcrTx2

Venom_Taxon_Group

sea anemone