TPDB

3D PDB MODEL

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TPDB ID TPDB72734

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 36-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

DCRWFLGGCSKDSDCCKHLACRIDGYIKYCAWDGTF

FASTA

>TPDB72734|Neurotoxin|length=36
DCRWFLGGCSKDSDCCKHLACRIDGYIKYCAWDGTF

Formula C180H263N49O52S6

Absent Amino Acids NEQMPV

Common Amino Acids C

Mass 4137.73

PI 6.75

Basic Residues 6

Acidic Residues 5

Hydrophobic Residues 12

Net Charge -0

Boman Index 0.78

Hydrophobicity -0.247

Aliphatic Index 48.89

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: >10 hour

Extinction Coefficient Cystines 14355

Absorbance 280nm 346.93

Polar Residues 11

number

2

A

2

R

0

N

5

D

6

C

0

E

0

Q

4

G

1

H

2

I

2

L

3

K

0

M

2

F

0

P

2

S

1

T

2

W

2

Y

0

V

Amino Acid Distribution

A: 2 R: 2 N: 0 D: 5 C: 6 E: 0 Q: 0 G: 4 H: 1 I: 2 L: 2 K: 3 M: 0 F: 2 P: 0 S: 2 T: 1 W: 2 Y: 2 V: 0

Length

36

Formula

C180H263N49O52S6

Mass

4137.73

PI

6.75

Net Charge

-0

Boman Index

0.78

Hydrophobicity

-0.247

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Neurotoxin that selectively increases Nav1.8/SCN10A peak current (EC(50)=894 nM) and causes a large hyperpolarising shift in both the voltage-dependence of activation and steady-state fast inactivation. Acts by binding to the domain II S3-S4 extracellular loop of the channel. In vivo, causes spontaneous pain behaviors (that gradually subsides over 30 minutes) when intraplantarly injected into mice. The same injection in Nav1.8/SCN10A knockout mice shows a reduction in pain behaviors, but not abolition, suggesting that activity on other Nav channels likely contributes to the nociceptive activity. {ECO:0000269|PubMed:35095499}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Encyocratella olivacea (Tanzanian black and olive baboon spider)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Neurotoxin that selectively increases Nav1.8/SCN10A peak current (EC(50)=894 nM) and causes a large hyperpolarising shift in both the voltage-dependence of activation and steady-state fast inactivation. Acts by binding to the domain II S3-S4 extracellular loop of the channel.

Protein Name Beta-theraphotoxin-Eo1a (Beta-TRTX-Eo1a)

Venom Taxon Group spider

Protein Family Neurotoxin 10 (Hwtx-1) family, 62 (Vatx) subfamily

Protein Existence Evidence at protein level

Keywords Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:35095499}.

Keywords

Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 10 (Hwtx-1) family, 62 (Vatx) subfamily

Protein_Name

Beta-theraphotoxin-Eo1a (Beta-TRTX-Eo1a)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:35095499}.

Venom_Taxon_Group

spider