TPDB

3D PDB MODEL

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TPDB ID TPDB72733

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 39-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

GVDKEGCRYLLGACTIDDDCCLHLGCNKKYGHCGWDGTF

FASTA

>TPDB72733|Neurotoxin|length=39
GVDKEGCRYLLGACTIDDDCCLHLGCNKKYGHCGWDGTF

Formula C181H273N51O57S6

Absent Amino Acids QMPS

Common Amino Acids G

Mass 4267.83

PI 5.23

Basic Residues 6

Acidic Residues 6

Hydrophobic Residues 11

Net Charge -2

Boman Index 5.69

Hydrophobicity -0.290

Aliphatic Index 60.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 8855

Absorbance 280nm 207.48

Polar Residues 11

number

1

A

1

R

1

N

5

D

6

C

1

E

0

Q

7

G

2

H

1

I

4

L

3

K

0

M

1

F

0

P

0

S

2

T

1

W

2

Y

1

V

Amino Acid Distribution

A: 1 R: 1 N: 1 D: 5 C: 6 E: 1 Q: 0 G: 7 H: 2 I: 1 L: 4 K: 3 M: 0 F: 1 P: 0 S: 0 T: 2 W: 1 Y: 2 V: 1

Length

39

Formula

C181H273N51O57S6

Mass

4267.83

PI

5.23

Net Charge

-2

Boman Index

5.69

Hydrophobicity

-0.290

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Insecticidal toxin that acts, at least partially, by inhibiting insect voltage-gated sodium (NaV) channels of several insect species. The toxin binds to the voltage sensor in NaV channel domain II and inhibits channel opening by shifting the threshold for channel activation to more positive voltages. The toxin binding is sensitive to residues in the S1-S2 loop of the domain II voltage sensor. In vivo, the recombinant toxin causes paralysis and/or death to two dipteran species (Lucilia cuprina and Drosophila melanogaster). In contrast, the toxin does not show paralytic or lethal effect on the cotton bollworm Helicoverpa armigera and the triatomine bug Rhodinius prolixus. {ECO:0000269|PubMed:27383378}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Augacephalus ezendami (Mozambique baboon spider) (Ceratogyrus ezendami)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Insecticidal toxin that acts, at least partially, by inhibiting insect voltage-gated sodium (NaV) channels of several insect species. The toxin binds to the voltage sensor in NaV channel domain II and inhibits channel opening by shifting the threshold for channel activation to more positive voltages.

Protein Name Mu-theraphotoxin-Ae1a (Mu-TRTX-Ae1a)

Venom Taxon Group spider

Protein Family Neurotoxin 10 (Hwtx-1) family, 47 subfamily

Protein Existence Evidence at protein level

Keywords Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:27383378}.

Keywords

Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 10 (Hwtx-1) family, 47 subfamily

Protein_Name

Mu-theraphotoxin-Ae1a (Mu-TRTX-Ae1a)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:27383378}.

Venom_Taxon_Group

spider