TPDB

3D PDB MODEL

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TPDB ID TPDB72726

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 37-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

FDGRNAAGNDKMSALMALTTRGCCSHPVCSAMSPICG

FASTA

>TPDB72726|Neurotoxin|length=37
FDGRNAAGNDKMSALMALTTRGCCSHPVCSAMSPICG

Formula C152H250N48O50S7

Absent Amino Acids EQWY

Common Amino Acids A

Mass 3774.37

PI 7.85

Basic Residues 4

Acidic Residues 2

Hydrophobic Residues 13

Net Charge +1

Boman Index 3.62

Hydrophobicity 0.116

Aliphatic Index 52.97

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 250

Absorbance 280nm 6.62

Polar Residues 12

number

5

A

2

R

2

N

2

D

4

C

0

E

0

Q

4

G

1

H

1

I

2

L

1

K

3

M

1

F

2

P

4

S

2

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 5 R: 2 N: 2 D: 2 C: 4 E: 0 Q: 0 G: 4 H: 1 I: 1 L: 2 K: 1 M: 3 F: 1 P: 2 S: 4 T: 2 W: 0 Y: 0 V: 1

Length

37

Formula

C152H250N48O50S7

Mass

3774.37

PI

7.85

Net Charge

+1

Boman Index

3.62

Hydrophobicity

0.116

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin inhibits alpha-3-beta-4/CHRNA3-CHRNB4 (IC(50)=3.6-18.38 nM), alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC(50)=33.9-94.1 nM), and alpha-2-beta-4/CHRNA2-CHRNB4 (IC(50)=4550 nM) nAChRs (PubMed:24200193, PubMed:28603989, PubMed:29925760). The toxin competes with agonists in the orthosteric binding site of alpha-3-beta-4/CHRNA3-CHRNB4 and alpha-6-beta-4/CHRNA6-CHRNB4 (PubMed:30252466). {ECO:0000269|PubMed:24200193, ECO:0000269|PubMed:28603989, ECO:0000269|PubMed:29925760, ECO:0000305|PubMed:30252466}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus textile (Cloth-of-gold cone)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin

Mechanism

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin inhibits alpha-3-beta-4/CHRNA3-CHRNB4 (IC(50)=3.6-18.38 nM), alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC(50)=33.9-94.1 nM), and alpha-2-beta-4/CHRNA2-CHRNB4 (IC(50)=4550 nM) nAChRs (PubMed:24200193, PubMed:28603989, PubMed:29925760).

Protein Name Alpha-conotoxin TxID (Alpha-conotoxin TxIC)

Venom Taxon Group cone snail

Protein Family Conotoxin A superfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Acetylcholine receptor inhibiting toxin Amidation Disulfide bond Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:24200193}.

Keywords

3D-structure Acetylcholine receptor inhibiting toxin Amidation Disulfide bond Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin A superfamily

Protein_Name

Alpha-conotoxin TxID (Alpha-conotoxin TxIC)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:24200193}.

Venom_Taxon_Group

cone snail