TPDB

3D PDB MODEL

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TPDB ID TPDB72707

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 74-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MRAIISLLLISTMVFGVIEAVSLEEGLKIFEGERGDCVGESQQCADWSGPYCCKGYYCTCRYFPKCICVNDNGK

FASTA

>TPDB72707|Neurotoxin|length=74
MRAIISLLLISTMVFGVIEAVSLEEGLKIFEGERGDCVGESQQCADWSGPYCCKGYYCTCRYFPKCICVNDNGK

Formula C359H560N92O108S10

Absent Amino Acids H

Common Amino Acids CG

Mass 8213.55

PI 4.69

Basic Residues 7

Acidic Residues 9

Hydrophobic Residues 29

Net Charge -2

Boman Index 9.91

Hydrophobicity 0.164

Aliphatic Index 81.62

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 11960

Absorbance 280nm 145.61

Polar Residues 23

number

3

A

3

R

2

N

3

D

8

C

6

E

2

Q

8

G

0

H

6

I

5

L

4

K

2

M

3

F

2

P

5

S

2

T

1

W

4

Y

5

V

Amino Acid Distribution

A: 3 R: 3 N: 2 D: 3 C: 8 E: 6 Q: 2 G: 8 H: 0 I: 6 L: 5 K: 4 M: 2 F: 3 P: 2 S: 5 T: 2 W: 1 Y: 4 V: 5

Length

74

Formula

C359H560N92O108S10

Mass

8213.55

PI

4.69

Net Charge

-2

Boman Index

9.91

Hydrophobicity

0.164

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Insecticidal neurotoxin that modulates the insect Nav channel (DmNaV1/tipE (para/tipE)) in a unique manner, with both the activation and inactivation processes being affected. The voltage dependence of activation is shifted toward more hyperpolarized potentials (analogous to site 4 toxins) and a non-inactivating persistent sodium current is induced (site 3-like action). Interestingly, both effects take place in a voltage-dependent manner, producing a bell-shaped curve between -80 and 0 mV. In vivo, induces an irreversible spastic paralysis when injected into insects. {ECO:0000269|PubMed:20385552}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Agelena orientalis (Funnel-web spider)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Insecticidal neurotoxin that modulates the insect Nav channel (DmNaV1/tipE (para/tipE)) in a unique manner, with both the activation and inactivation processes being affected. The voltage dependence of activation is shifted toward more hyperpolarized potentials (analogous to site 4 toxins) and a non-inactivating persistent sodium current is induced (site 3-like action).

Protein Name U3-agatoxin-Ao1f (U3-AGTX-Ao1f) (Beta/delta-agatoxin-1) (Mu-2Aga_07)

Venom Taxon Group spider

Protein Family Neurotoxin 07 (Beta/delta-agtx) family, 03 (aga-4) subfamily, Aga sub-subfamily

Protein Existence Evidence at protein level

Keywords Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:20385552}.

Keywords

Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 07 (Beta/delta-agtx) family, 03 (aga-4) subfamily, Aga sub-subfamily

Protein_Name

U3-agatoxin-Ao1f (U3-AGTX-Ao1f) (Beta/delta-agatoxin-1) (Mu-2Aga_07)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:20385552}.

Venom_Taxon_Group

spider