TPDB

3D PDB MODEL

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TPDB ID TPDB72638

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 87-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MNSLLMITACFFLIGTVWAKEGYLVNKSTGCKYGCLLLGKNEGCDKECKAKNQGGSYGYCYAFGCWCEGLPESTPTYPLPNKSCSKK

FASTA

>TPDB72638|Neurotoxin|length=87
MNSLLMITACFFLIGTVWAKEGYLVNKSTGCKYGCLLLGKNEGCDKECKAKNQGGSYGYCYAFGCWCEGLPESTPTYPLPNKSCSKK

Formula C422H649N105O123S11

Absent Amino Acids RH

Common Amino Acids G

Mass 9514.08

PI 8.20

Basic Residues 10

Acidic Residues 6

Hydrophobic Residues 30

Net Charge +4

Boman Index 5.75

Hydrophobicity -0.186

Aliphatic Index 60.57

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 20440

Absorbance 280nm 214.84

Polar Residues 32

number

4

A

0

R

5

N

1

D

9

C

5

E

1

Q

11

G

0

H

2

I

9

L

10

K

2

M

3

F

4

P

6

S

5

T

2

W

6

Y

2

V

Amino Acid Distribution

A: 4 R: 0 N: 5 D: 1 C: 9 E: 5 Q: 1 G: 11 H: 0 I: 2 L: 9 K: 10 M: 2 F: 3 P: 4 S: 6 T: 5 W: 2 Y: 6 V: 2

Length

87

Formula

C422H649N105O123S11

Mass

9514.08

PI

8.20

Net Charge

+4

Boman Index

5.75

Hydrophobicity

-0.186

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is lethal to crustaceans (freshwater crayfish (Cambarellus montezumae spp.)), it provokes a reversible paralysis to insects (crickets (Achaeta spp.)), but is not toxic to mice. At high concentrations, it does displace the (beta) mammal-specific toxin Cn2 from rat brain synaptosomes. {ECO:0000269|PubMed:19631296}.

Target

Target or Channel voltage-gated sodium channel (Nav) proton-gated sodium channel / ASIC

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Centruroides suffusus (Durango bark scorpion)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is lethal to crustaceans (freshwater crayfish (Cambarellus montezumae spp.)), it provokes a reversible paralysis to insects (crickets (Achaeta spp.)), but is not toxic to mice.

Protein Name Toxin Css39.8

Venom Taxon Group scorpion

Protein Family Long (4 C-C) scorpion toxin superfamily, Sodium channel inhibitor family, Beta subfamily

Protein Existence Evidence at protein level

Keywords Cleavage on pair of basic residues Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:19631296}.

Keywords

Cleavage on pair of basic residues Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Long (4 C-C) scorpion toxin superfamily, Sodium channel inhibitor family, Beta subfamily

Protein_Name

Toxin Css39.8

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:19631296}.

Venom_Taxon_Group

scorpion