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Peptide record

TPDB72540

Neurotoxin standard
40 amino acids
Basic Information
3D PDB MODEL
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TPDB72540
Neurotoxin
Toxicity and safety peptides
NTxPred2
standard
No
A 40-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.
Sequence
CGDINAPCQSDCDCCGYSVTCDCYWGNECKCRESNFAIGM
Physicochemical Analysis
C174H259N49O63S10
HL
C
4365.86
3.69
2
6
10
-4
5.48
-0.230
31.75
Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour
8980
205.69
19
Residue Composition
number
2
A
1
R
3
N
4
D
9
C
2
E
1
Q
4
G
0
H
2
I
0
L
1
K
1
M
1
F
1
P
3
S
1
T
1
W
2
Y
1
V
Amino Acid Distribution
A: 2 R: 1 N: 3 D: 4 C: 9 E: 2 Q: 1 G: 4 H: 0 I: 2 L: 0 K: 1 M: 1 F: 1 P: 1 S: 3 T: 1 W: 1 Y: 2 V: 1
Chemical Descriptors
40
C174H259N49O63S10
4365.86
3.69
-4
5.48
-0.230
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Potent insecticidal toxin that binds to two distinct sites in insect sodium channels, with close affinity (Kd1=34.7 pM and Kd2=35.1 pM) (PubMed:26220799). Its association is rather fast (1.4 and 8.5 minutes, respectively for sites 1 and 2) and its dissociation is a slower process (5.4 and 32.8 minutes, respectively) (PubMed:26220799). On rat brain synaptosomes the toxin partially competes (~30%) with the beta-toxin CssIV, but does not compete with the alpha-toxin AaII, nor with the beta-toxin Ts VII (PubMed:26220799). On cockroach nerve cord synaptosomes, the toxin does not compete with the anti-insect toxin LqqIT1, but it competes with the 'alpha-like' toxin BomIV (IC(50)=80 pM) (PubMed:26220799). In cockroach neurons, the toxin inhibits the inactivation of sodium channels and it shifts the sodium channel activation to hyperpolarizing potentials (PubMed:26220799). Hence, it behaves like an 'alpha-like' toxin and binds preferentially to site 3 on the insect Nav channel, located on the domain IV (PubMed:26220799). The toxin may also inhibit the N-methyl-D-aspartate (NMDA)-subtype of ionotropic glutamate receptor (GRIN) (By similarity). In vivo, the toxin causes excitatory effects on insects (PubMed:26220799). {ECO:0000250|UniProtKB:P59367, ECO:0000269|PubMed:26220799}.

Target

voltage-gated sodium channel (Nav) glutamate receptor

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Phoneutria reidyi (Brazilian Amazonian armed spider) (Ctenus reidyi)
Neurotoxin Postsynaptic neurotoxin Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Voltage-gated sodium channel impairing toxin
Potent insecticidal toxin that binds to two distinct sites in insect sodium channels, with close affinity (Kd1=34.7 pM and Kd2=35.1 pM) (PubMed:26220799). Its association is rather fast (1.4 and 8.5 minutes, respectively for sites 1 and 2) and its dissociation is a slower process (5.4 and 32.8 minutes, respectively) (PubMed:26220799).
Beta/delta-ctenitoxin-Pr1a (Beta/delta-CNTX-Pr1a) (Beta/delta-PrIT1)
spider
Neurotoxin 03 (Tx2) family, 05 subfamily
Evidence at protein level
Direct protein sequencing Disulfide bond Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Knottin Neurotoxin Postsynaptic neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:26220799}.
Additional Detail Fields 7 fields
Direct protein sequencing Disulfide bond Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Knottin Neurotoxin Postsynaptic neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Postsynaptic neurotoxin Ion channel impairing toxin Ionotropic glutamate receptor inhibitor Voltage-gated sodium channel impairing toxin
Evidence at protein level
Neurotoxin 03 (Tx2) family, 05 subfamily
Beta/delta-ctenitoxin-Pr1a (Beta/delta-CNTX-Pr1a) (Beta/delta-PrIT1)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:26220799}.
spider