TPDB

3D PDB MODEL

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TPDB ID TPDB72528

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 49-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

KGGYPVDSKGCKISCVINNEYCSRDCTSGYCYFLRWGLACWCDGVPPQR

FASTA

>TPDB72528|Neurotoxin|length=49
KGGYPVDSKGCKISCVINNEYCSRDCTSGYCYFLRWGLACWCDGVPPQR

Formula C239H358N66O70S7

Absent Amino Acids HM

Common Amino Acids C

Mass 5500.29

PI 7.92

Basic Residues 6

Acidic Residues 4

Hydrophobic Residues 15

Net Charge +2

Boman Index 2.63

Hydrophobicity -0.337

Aliphatic Index 51.63

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 17335

Absorbance 280nm 315.17

Polar Residues 19

number

1

A

3

R

2

N

3

D

7

C

1

E

1

Q

6

G

0

H

2

I

2

L

3

K

0

M

1

F

3

P

4

S

1

T

2

W

4

Y

3

V

Amino Acid Distribution

A: 1 R: 3 N: 2 D: 3 C: 7 E: 1 Q: 1 G: 6 H: 0 I: 2 L: 2 K: 3 M: 0 F: 1 P: 3 S: 4 T: 1 W: 2 Y: 4 V: 3

Length

49

Formula

C239H358N66O70S7

Mass

5500.29

PI

7.92

Net Charge

+2

Boman Index

2.63

Hydrophobicity

-0.337

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:32973807). This toxin acts on X.laevis Nav1.6/SCN8A and insect BgNav1 channels, and also displays a small but significant effect on X.laevis Nav1.4/SCN4A channels (PubMed:32973807). In mice induces nociception (licking and lifting behaviors) during the first 15 minutes after injection, and increases the release of TNF in J774.1 cells (PubMed:32973807). {ECO:0000269|PubMed:32973807}.

Target

Target or Channel voltage-gated sodium channel (Nav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Rhopalurus crassicauda (Scorpion)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Mechanism

Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (PubMed:32973807). This toxin acts on X.laevis Nav1.6/SCN8A and insect BgNav1 channels, and also displays a small but significant effect on X.laevis Nav1.4/SCN4A channels (PubMed:32973807).

Protein Name Beta-toxin Rc1

Venom Taxon Group scorpion

Protein Family Long (4 C-C) scorpion toxin superfamily, Sodium channel inhibitor family, Beta subfamily

Protein Existence Evidence at protein level

Keywords Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:32973807}.

Keywords

Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Secreted Toxin Voltage-gated sodium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Long (4 C-C) scorpion toxin superfamily, Sodium channel inhibitor family, Beta subfamily

Protein_Name

Beta-toxin Rc1

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:32973807}.

Venom_Taxon_Group

scorpion