TPDB

3D PDB MODEL

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TPDB ID TPDB72520

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 42-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

ASDGRNAAADDKASDPIALTVRGGCCSHPVCYTKNPNCGGRR

FASTA

>TPDB72520|Neurotoxin|length=42
ASDGRNAAADDKASDPIALTVRGGCCSHPVCYTKNPNCGGRR

Formula C173H283N61O60S4

Absent Amino Acids EQMFW

Common Amino Acids A

Mass 4305.76

PI 8.30

Basic Residues 7

Acidic Residues 4

Hydrophobic Residues 11

Net Charge +2

Boman Index 10.78

Hydrophobicity -0.664

Aliphatic Index 46.67

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1740

Absorbance 280nm 40.41

Polar Residues 13

number

6

A

4

R

3

N

4

D

4

C

0

E

0

Q

5

G

1

H

1

I

1

L

2

K

0

M

0

F

3

P

3

S

2

T

0

W

1

Y

2

V

Amino Acid Distribution

A: 6 R: 4 N: 3 D: 4 C: 4 E: 0 Q: 0 G: 5 H: 1 I: 1 L: 1 K: 2 M: 0 F: 0 P: 3 S: 3 T: 2 W: 0 Y: 1 V: 2

Length

42

Formula

C173H283N61O60S4

Mass

4305.76

PI

8.30

Net Charge

+2

Boman Index

10.78

Hydrophobicity

-0.664

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (PubMed:31255696). This toxin potently and selectively inhibits human and rat alpha-6-beta-4/CHRNA6-CHRNB4 nAChR (IC(50)=12 nM on rat nAChR) (PubMed:31255696). It exhibits rapid binding and unbinding at this receptor (PubMed:31255696). It also shows activity on rat alpha-6-beta-4/CHRNA6-CHRNB4 (IC(50)=12 nM), human alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC(50)=5.3 nM), rat alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC(50)=18 nM), rat alpha-3-beta-4/CHRNA3-CHRNB4 (IC(50)=320 nM), and rat alpha-6/alpha-3-beta-2-beta-3 (CHRNA6/CHRNA3-CHRNB2-CHRNB3) (IC(50)=4 uM) (PubMed:31255696). {ECO:0000269|PubMed:31255696}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus ventricosus (Mediterranean cone)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin

Mechanism

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (PubMed:31255696). This toxin potently and selectively inhibits human and rat alpha-6-beta-4/CHRNA6-CHRNB4 nAChR (IC(50)=12 nM on rat nAChR) (PubMed:31255696).

Protein Name Alpha-conotoxin VnIB

Venom Taxon Group cone snail

Protein Family Conotoxin A superfamily

Protein Existence Evidence at protein level

Keywords Acetylcholine receptor inhibiting toxin Amidation Disulfide bond Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:31255696}.

Keywords

Acetylcholine receptor inhibiting toxin Amidation Disulfide bond Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin A superfamily

Protein_Name

Alpha-conotoxin VnIB

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:31255696}.

Venom_Taxon_Group

cone snail